Department of Orthopaedic Surgery, Hanyang University Hospital, Seoul 04763, Korea.
Institute for Rheumatology Research, Hanyang University, Seoul 04763, Korea.
Cells. 2021 Mar 27;10(4):740. doi: 10.3390/cells10040740.
Muscle tissue is often removed during hamstring tendon graft preparation for anterior cruciate ligament (ACL) reconstruction. The purpose of the study was to test whether preservation of muscle remnants on a tendon graft is beneficial to the graft healing process following ACL reconstruction. Co-culturing of tendon-derived cells (TDCs) and muscle-derived cells (MDCs) was performed at various ratios, and their potential for cell viability and multilineage differentiation was compared to a single TDC cell group. Ligamentous and chondrogenic differentiation was most enhanced when a small population of MDCs was co-cultured with TDCs (6:2 co-culture group). Cell viability and osteogenic differentiation were proportionally enhanced with increasing MDC population size. MDCs co-cultured with TDCs possess both the ability to enhance cell viability and differentiate into other cell lineages.
在进行前交叉韧带（ACL）重建的腘绳肌腱移植物准备时，通常会切除肌肉组织。本研究的目的是测试在 ACL 重建后，保留肌腱移植物上的肌肉残端是否有利于移植物愈合过程。在不同比例下进行肌腱源性细胞（TDC）和肌源性细胞（MDC）的共培养，并将其细胞活力和多向分化潜能与单个 TDC 细胞组进行比较。当少量 MDC 与 TDC 共培养时，韧带和成软骨分化得到最大增强（6:2 共培养组）。随着 MDC 群体规模的增加，细胞活力和成骨分化呈比例增强。与 TDC 共培养的 MDC 具有增强细胞活力和分化为其他细胞谱系的能力。
