Liu Zhuo, Feng Xiangli, Wang Xiangyao, Yang Shiyuan, Mao Jing, Gong Shiqiang
Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
Materials (Basel). 2021 Mar 3;14(5):1178. doi: 10.3390/ma14051178.
Bacterial reinfection and root fracture are the main culprits related to root canal treatment failure. This study aimed to assess the utility of quercetin solution as an adjunctive endodontic irrigant that does not weaken root canal dentin with commitment anti-biofilm activity and bio-safety. Based on a noninvasive dentin infection model, dentin tubules infected with () were irrigated with sterile water (control group), and 0, 1, 2, 4 wt% quercetin-containing ethanol solutions. Live and dead bacteria percentages in biofilms were analyzed by confocal laser scanning microscopy (CLSM). Elastic modulus, hydroxyproline release and X-ray photoelectron spectroscopy (XPS) characterization were tested to evaluate the irrigants' collagen-stabilizing effect. The cytotoxicity was tested by CCK-8 assay. Quercetin increased the proportion of dead bacteria volumes within and improved the flexural strength of dentin compared to control group ( < 0.05). Quercetin-treated dentin matrix had less elasticity loss and hydroxyproline release after collagenase degradation ( < 0.05). Moreover, quercetin solutions revealed an increase in the C-O peak area under both C1s and O1s narrow-scan spectra of XPS characterization, and no cytotoxicity ( > 0.05). Quercetin exhibited anti-biofilm activity, a collagen-stabilizing effect with cytocompatibility, supporting quercetin as a potential candidate for endodontic irrigant.
细菌再感染和牙根折断是与根管治疗失败相关的主要原因。本研究旨在评估槲皮素溶液作为一种辅助性牙髓冲洗剂的效用，该冲洗剂不会削弱根管牙本质，同时具有可靠的抗生物膜活性和生物安全性。基于一种非侵入性牙本质感染模型，用无菌水（对照组）以及含0、1、2、4 wt%槲皮素的乙醇溶液冲洗感染了（）的牙本质小管。通过共聚焦激光扫描显微镜（CLSM）分析生物膜中活菌和死菌的百分比。测试弹性模量、羟脯氨酸释放量以及X射线光电子能谱（XPS）表征，以评估冲洗剂对胶原蛋白的稳定作用。通过CCK-8法检测细胞毒性。与对照组相比，槲皮素增加了（）内死菌体积的比例，并提高了牙本质的抗弯强度（<0.05）。经槲皮素处理的牙本质基质在胶原酶降解后弹性损失和羟脯氨酸释放较少（<0.05）。此外，槲皮素溶液在XPS表征的C1s和O1s窄扫描光谱下C-O峰面积增加，且无细胞毒性（>0.05）。槲皮素具有抗生物膜活性、对胶原蛋白的稳定作用以及细胞相容性，支持槲皮素作为牙髓冲洗剂的潜在候选物。
