Fernandes Camila, Martins Leonor, Teixeira Miguel, Blom Jochen, Pothier Joël F, Fonseca Nuno A, Tavares Fernando
CIBIO-Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO-Laboratório Associado, Universidade do Porto, Rua Padre Armando Quintas 7, 4485-661 Vairão, Portugal.
FCUP-Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal.
Microorganisms. 2021 Mar 17;9(3):624. doi: 10.3390/microorganisms9030624.
The recent report of distinct lineages of pv. and within the same walnut tree revealed that this consortium of walnut-associated includes both pathogenic and nonpathogenic strains. As the implications of this co-colonization are still poorly understood, in order to unveil niche-specific adaptations, the genomes of three strains (CPBF 367, CPBF 424, and CPBF 426) and of an pv. strain (CPBF 427) isolated from a single walnut tree in Loures (Portugal) were sequenced with two different technologies, Illumina and Nanopore, to provide consistent single scaffold chromosomal sequences. General genomic features showed that CPBF 427 has a genome similar to other pv. strains, regarding its size, number, and content of CDSs, while strains show a reduction regarding these features comparatively to pv. strains. Whole genome comparisons revealed remarkable genomic differences between pv. and strains, which translates into different pathogenicity and virulence features, namely regarding type 3 secretion system and its effectors and other secretory systems, chemotaxis-related proteins, and extracellular enzymes. Altogether, the distinct genomic repertoire of may be particularly useful to address pathogenicity emergence and evolution in walnut-associated .
最近关于同一棵核桃树中不同谱系的青枯雷尔氏菌（Ralstonia solanacearum pv. solanacearum）和青枯雷尔氏菌（Ralstonia solanacearum）的报告显示，这种与核桃相关的菌群包括致病菌株和非致病菌株。由于这种共定殖的影响仍知之甚少，为了揭示特定生态位的适应性，对从葡萄牙洛雷斯的一棵核桃树中分离出的三株青枯雷尔氏菌（Ralstonia solanacearum）菌株（CPBF 367、CPBF 424和CPBF 426）以及一株青枯雷尔氏菌（Ralstonia solanacearum pv. solanacearum）菌株（CPBF 427）的基因组，使用Illumina和Nanopore两种不同技术进行了测序，以提供一致且无间隙的染色体序列。一般基因组特征表明，CPBF 427的基因组在大小、编码序列（CDS）数量和内容方面与其他青枯雷尔氏菌（Ralstonia solanacearum pv. solanacearum）菌株相似，而青枯雷尔氏菌（Ralstonia solanacearum）菌株在这些特征上相对于青枯雷尔氏菌（Ralstonia solanacearum pv. solanacearum）菌株有所减少。全基因组比较揭示了青枯雷尔氏菌（Ralstonia solanacearum pv. solanacearum）和青枯雷尔氏菌（Ralstonia solanacearum）菌株之间显著的基因组差异，这转化为不同的致病性和毒力特征，特别是在Ⅲ型分泌系统及其效应子、其他分泌系统、趋化相关蛋白和细胞外酶方面。总之，青枯雷尔氏菌（Ralstonia solanacearum）独特的基因组库可能对研究核桃相关青枯雷尔氏菌（Ralstonia solanacearum）的致病性出现和进化特别有用。
