Râpă Maria, Gaidău Carmen, Stefan Laura Mihaela, Matei Ecaterina, Niculescu Mihaela, Berechet Mariana Daniela, Stanca Maria, Tablet Cristina, Tudorache Mădălina, Gavrilă Raluca, Predescu Cristian, Vidu Ruxandra
Faculty of Material Sciences and Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania.
National Research and Development Institute for Textiles and Leather- Division Leather and Footwear Research Institute, 031215 Bucharest, Romania.
Materials (Basel). 2020 Jul 15;13(14):3149. doi: 10.3390/ma13143149.
Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG), and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FTIR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Furthermore, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both in vitro quantitative and qualitative analysis, the electrospun nanofibers were not cytotoxic, inducing a healthy cellular response. The results of new electrospun protein-based nanofibers may be useful for further research on bioactive properties of these nanofibers for tissue engineering.
对浓缩胶原蛋白水解物（HC10CC）、兔胶原蛋白胶（RCG）和角蛋白水解物（KH）进行了研究，涉及从哺乳动物副产品中提取以及通过静电纺丝进行加工。通过扫描电子显微镜结合能量色散X射线光谱（SEM/EDS）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）、差示扫描量热法（DSC）和压痕测试对静电纺纳米纤维进行了表征。使用MTT和LDH测定法以及细胞形态观察，对L929成纤维细胞进行了静电纺纳米纤维的细胞毒性测试。静电纺RCG和KH纳米纤维的形态显示纳米纤维的平均尺寸在44至410纳米之间，而静电纺HC10CC纳米纤维的尺寸更大。对提取的蛋白质和静电纺纳米纤维进行的ATR-FTIR光谱表明，胶原蛋白的三螺旋结构部分得以保留。结果与蛋白质提取物的圆二色性分析一致。此外，静电纺KH纳米纤维的粘弹性性能优于静电纺RCG纳米纤维。基于体外定量和定性分析，静电纺纳米纤维无细胞毒性，可诱导健康的细胞反应。新型静电纺蛋白质基纳米纤维的结果可能有助于进一步研究这些纳米纤维在组织工程中的生物活性特性。
