Choi Soon-Won, Kim Yoon-Hwan, Kang Min Soo, Jeong Yunho, Ahn Jin-Ok, Choi Jung Hoon, Chung Jin-Young
Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si 24341, Korea.
Department of Veterinary Anatomy and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si 24341, Korea.
Vet Sci. 2021 Mar 18;8(3):51. doi: 10.3390/vetsci8030051.
Acute pancreatitis is an acute inflammatory process in the pancreas that is common in dogs. This study was designed to compare cytokines between healthy dogs and dogs with suspected acute pancreatitis. For the canine cytokine antibody array, three healthy dogs and three dogs with suspected acute pancreatitis were included. Interleukin (IL)-2, IL-6, IL-10, GM-CSF, and TNF-α were not detected in either group based on the results. Conversely, IL-8 ( = 0.035), Monocyte Chemoattractant Protein-1 (MCP)-1 ( = 0.0138), Receptor for Advanced Glycation Endproducts (RAGE) ( = 0.0079), and stem cell factor (SCF) ( = 0.034) were significantly increased in dogs with suspected acute pancreatitis. However, vascular endothelial growth factor (VEGF) ( = 0.6971) did not differ significantly between groups. For the canine serum Enzyme-Linked Immunosorbent Assay (ELISA), eight healthy dogs and eight dogs with suspected acute pancreatitis were included. ELISA revealed that IL-8 ( < 0.0001), MCP-1 ( < 0.0001), RAGE ( = 0.006), and SCF ( = 0.0002) were all significantly upregulated in the experimental group. We confirmed multiple patterns of cytokines in suspected acute pancreatitis of dogs via canine cytokine antibody array using a small quantity of serum. After this procedure, we reevaluated the cytokines, which were significantly increased in dogs with suspected acute pancreatitis, by ELISA, with more samples. Through this study, we confirmed that MCP-1, RAGE, and SCF were newly suggested factors in dogs with suspected acute pancreatitis.
急性胰腺炎是犬类常见的胰腺急性炎症过程。本研究旨在比较健康犬与疑似急性胰腺炎犬的细胞因子。对于犬细胞因子抗体阵列，纳入了3只健康犬和3只疑似急性胰腺炎犬。根据结果，两组均未检测到白细胞介素（IL）-2、IL-6、IL-10、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和肿瘤坏死因子-α（TNF-α）。相反，疑似急性胰腺炎犬的IL-8（=0.035）、单核细胞趋化蛋白-1（MCP）-1（=0.0138）、晚期糖基化终产物受体（RAGE）（=0.0079）和干细胞因子（SCF）（=0.034）显著升高。然而，血管内皮生长因子（VEGF）（=0.6971）在两组之间无显著差异。对于犬血清酶联免疫吸附测定（ELISA），纳入了8只健康犬和8只疑似急性胰腺炎犬。ELISA显示，实验组中IL-8（<0.0001）、MCP-1（<0.0001）、RAGE（=0.006）和SCF（=0.0002）均显著上调。我们通过使用少量血清的犬细胞因子抗体阵列证实了犬疑似急性胰腺炎中细胞因子的多种模式。在此过程之后，我们通过ELISA用更多样本重新评估了在疑似急性胰腺炎犬中显著升高的细胞因子。通过本研究，我们证实MCP-1、RAGE和SCF是疑似急性胰腺炎犬中新发现的因子。
