Condurache Lazăr Nina-Nicoleta, Croitoru Constantin, Enachi Elena, Bahrim Gabriela-Elena, Stănciuc Nicoleta, Râpeanu Gabriela
Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, 111 Domneasca Street, E Building, 800201 Galati, Romania.
Academy of Agricultural and Forestry Sciences, 61 Marasti Blvd, 011464 Bucharest, Romania.
Plants (Basel). 2021 Mar 18;10(3):577. doi: 10.3390/plants10030577.
This study aimed to use eggplant peels as a potential source of anthocyanins with biological activities. Two different extraction methods were tested in order to obtain extracts with a high anthocyanin content. The selected methods were the solid-liquid extraction (SLE) and ultrasound-assisted extraction (UAE) methods. For each method, two concentrations of ethanol (EtOH) were used, while varying the extraction time and temperature. Based on the results, the extracts obtained by SLE using EtOH 96% after 30 min of extraction at 50 °C showed the highest anthocyanin concentration. The UAE allowed the best results with EtOH 96% after 30 min at 25 °C. Both selected extracts showed similar chromatographic profiles, with delphinidin 3--rutinoside as the major anthocyanin, but in a higher concentration in UAE. The extracts also presented inhibitory activity against lipoxygenase (LOX), lipase, and α-amylase, thus suggesting a possible involvement in reducing the risk of various disorders. The first order kinetic model was used to predict the changes that can occur in the anthocyanin content and antioxidant activity from the eggplant peel extract. The calculated kinetic and thermodynamic parameters confirm the irreversible degradation of phytochemicals.
本研究旨在将茄子皮作为具有生物活性的花青素潜在来源。为获得高花青素含量的提取物,测试了两种不同的提取方法。所选方法为固液萃取(SLE)和超声辅助萃取(UAE)方法。对于每种方法,使用了两种乙醇(EtOH)浓度,同时改变提取时间和温度。结果表明,在50℃下提取30分钟后,使用96%乙醇通过SLE获得的提取物花青素浓度最高。在25℃下30分钟后,使用96%乙醇的UAE效果最佳。两种所选提取物显示出相似的色谱图,矢车菊素3 - 芸香糖苷为主要花青素,但在UAE中浓度更高。提取物还对脂氧合酶(LOX)、脂肪酶和α -淀粉酶具有抑制活性,因此表明可能参与降低各种疾病的风险。采用一级动力学模型预测茄子皮提取物中花青素含量和抗氧化活性可能发生的变化。计算得到的动力学和热力学参数证实了植物化学物质的不可逆降解。