Department of Microbiology, School of Mathematical and Natural Sciences, University of Venda, Thohoyandou 0950, South Africa.
Viruses. 2021 Mar 1;13(3):393. doi: 10.3390/v13030393.
Diarrhoeal disease is considered an important cause of morbidity and mortality in developing areas, and a large contributor to the burden of disease in children younger than five years of age. This study investigated the prevalence and genogroups of human sapovirus (SV) in children ≤5 years of age in rural communities of Vhembe district, South Africa. Between 2017 and 2020, a total of 284 stool samples were collected from children suffering with diarrhoea ( = 228) and from children without diarrhoea ( = 56). RNA extraction using Boom extraction method, and screening for SV using real-time PCR were done in the lab. Positive samples were subjected to conventional RT-PCR targeting the capsid fragment. Positive sample isolates were genotyped using Sanger sequencing. Overall SV were detected in 14.1% (40/284) of the stool samples (16.7% (38/228) of diarrhoeal and 3.6% (2/56) of non-diarrhoeal samples). Significant correlation between SV positive cases and water sources was noted. Genogroup-I was identified as the most prevalent strain comprising 81.3% (13/16), followed by SV-GII 12.5% (2/16) and SV-GIV 6.2% (1/16). This study provides valuable data on prevalence of SV amongst outpatients in rural and underdeveloped communities, and highlights the necessity for further monitoring of SV circulating strains as potential emerging strains.
腹泻病被认为是发展中地区发病率和死亡率的重要原因，也是 5 岁以下儿童疾病负担的主要原因之一。本研究调查了南非威姆贝区农村社区≤5 岁儿童中人类杯状病毒（SV）的流行率和基因群。在 2017 年至 2020 年间，共收集了 284 份来自腹泻儿童（=228 份）和无腹泻儿童（=56 份）的粪便样本。在实验室中使用 Boom 提取方法提取 RNA，并使用实时 PCR 筛查 SV。对阳性样本进行针对衣壳片段的常规 RT-PCR。使用 Sanger 测序对阳性样本分离株进行基因分型。在 284 份粪便样本中，总体 SV 检出率为 14.1%（40/284）（腹泻样本为 16.7%（38/228），非腹泻样本为 3.6%（2/56））。注意到 SV 阳性病例与水源之间存在显著相关性。基因群-I 被鉴定为最常见的菌株，占 81.3%（13/16），其次是 SV-GII 12.5%（2/16）和 SV-GIV 6.2%（1/16）。本研究提供了关于农村和欠发达社区门诊患者中 SV 流行率的有价值数据，并强调了进一步监测 SV 循环株作为潜在新兴株的必要性。
