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益生菌MJ2通过提高骨保护素/核因子κB受体活化因子配体比值来增强成骨细胞分化和矿化。

Probiotic MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio.

作者信息

Yeom Jiah, Ma Seongho, Lim Young-Hee

机构信息

Department of Integrated Biomedical and Life Sciences, Graduate School, Korea University, Seoul 02841, Korea.

School of Biosystems and Biomedical Sciences, Korea University, Seoul 02841, Korea.

出版信息

Microorganisms. 2021 Mar 24;9(4):673. doi: 10.3390/microorganisms9040673.

DOI:10.3390/microorganisms9040673
PMID:33805153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8064112/
Abstract

Osteoblast differentiation is important for the development of bone and the maintenance of bone density. is a probiotic with an anti-inflammatory property. The aim of this study was to investigate the enhancement effect of MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, and its signaling pathway. For in vitro and in vivo experiments, human fetal osteoblastic cell line hFOB 1.19 and an ovariectomized rat model were used, respectively. Expression levels of genes and proteins related to osteoblast differentiation and mineralization were measured by real-time polymerase chain reaction (qPCR) and Western blotting, respectively. Alizarin red S staining was performed to measure osteoblast mineralization. Heat-killed MJ2 (hkMJ2)-treated cells showed significantly increased osteoblast differentiation via an increase in the osteoprotegerin (OPG)/receptor activator of nuclear factor-κB ligand (RANKL) ratio and significantly increased osteoblast mineralization by stimulating the expression of bone morphogenetic protein 2 and runt-related transcription factor 2. Additionally, oral administration of live or heat-killed MJ2 to ovariectomized rats inhibited osteoporosis-induced bone loss. Specifically, surface proteins isolated from MJ2 promoted osteoblast differentiation and mineralization. In conclusion, MJ2 enhanced osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway and the effective component of MJ2 might be its surface proteins.

摘要

成骨细胞分化对于骨骼发育和骨密度维持至关重要。[某物质]是一种具有抗炎特性的益生菌。本研究旨在探讨从生牛奶中分离出的MJ2对成骨细胞分化、矿化及其信号通路的增强作用。分别使用人胎儿成骨细胞系hFOB 1.19和去卵巢大鼠模型进行体外和体内实验。通过实时聚合酶链反应（qPCR）和蛋白质印迹法分别检测与成骨细胞分化和矿化相关的基因和蛋白质表达水平。进行茜素红S染色以测量成骨细胞矿化。经热灭活的MJ2（hkMJ2）处理的细胞通过增加骨保护素（OPG）/核因子κB受体活化因子配体（RANKL）比值，显著增强了成骨细胞分化，并通过刺激骨形态发生蛋白2和 runt相关转录因子2的表达，显著增加了成骨细胞矿化。此外，给去卵巢大鼠口服活的或热灭活的MJ2可抑制骨质疏松引起的骨质流失。具体而言，从MJ2中分离出的表面蛋白促进了成骨细胞分化和矿化。总之，MJ2通过OPG/RANKL信号通路增强了成骨细胞分化和矿化，MJ2的有效成分可能是其表面蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/8f3cc5034ac1/microorganisms-09-00673-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/e715cd79035c/microorganisms-09-00673-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/bfa131cb2b05/microorganisms-09-00673-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/c40b86b2516f/microorganisms-09-00673-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/f7148406f105/microorganisms-09-00673-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/dcb4c97f8b5e/microorganisms-09-00673-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/3c56e7663d5e/microorganisms-09-00673-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/952f7093416a/microorganisms-09-00673-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/07ccf6ce349d/microorganisms-09-00673-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/3a85410557a3/microorganisms-09-00673-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/a915d52b55e4/microorganisms-09-00673-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/8f3cc5034ac1/microorganisms-09-00673-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/e715cd79035c/microorganisms-09-00673-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/bfa131cb2b05/microorganisms-09-00673-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/c40b86b2516f/microorganisms-09-00673-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/f7148406f105/microorganisms-09-00673-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/dcb4c97f8b5e/microorganisms-09-00673-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/3c56e7663d5e/microorganisms-09-00673-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/952f7093416a/microorganisms-09-00673-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/07ccf6ce349d/microorganisms-09-00673-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/3a85410557a3/microorganisms-09-00673-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/a915d52b55e4/microorganisms-09-00673-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ff/8064112/8f3cc5034ac1/microorganisms-09-00673-g011.jpg

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