• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于液相色谱-质谱联用代谢组学从内生地衣真菌中发现潜在抗菌诊断代谢物

Biodiscovery of Potential Antibacterial Diagnostic Metabolites from the Endolichenic Fungus Using LC-MS-Based Metabolomics.

作者信息

Santiago Krystle Angelique A, Edrada-Ebel RuAngelie, Dela Cruz Thomas Edison E, Cheow Yuen Lin, Ting Adeline Su Yien

机构信息

School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Dahrul Ehsan 47500, Malaysia.

Tropical Medicine & Biology Multidisciplinary Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Dahrul Ehsan 47500, Malaysia.

出版信息

Biology (Basel). 2021 Mar 4;10(3):191. doi: 10.3390/biology10030191.

DOI:10.3390/biology10030191
PMID:33806264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8000601/
Abstract

Three species of the lichen ( (Stirt.) Zahlbr., Zahlbr. and Stirt.) and nine associated endolichenic fungi (ELF) were evaluated using a metabolomics approach. All investigated lichen crude extracts afforded antibacterial activity against (minimum inhibitory concentration (MIC): 0.0625 mg/mL), but none was observed against , while the ELF extract was found to be the most active against (MIC: 2.5 mg/mL) and (MIC: 5 mg/mL). was fractionated and tested for to determine its antibacterial activity. Fractions XvFr1 to 5 displayed bioactivities against both test bacteria. Selected crude extracts and fractions were subjected to metabolomics analyses using high-resolution LC-MS. Multivariate analyses showed the presence of five secondary metabolites unique to bioactive fractions XvFr1 to 3, which were identified as responsible for the antibacterial activity of The -values of these metabolites were at the margin of significance level, with methyl xylariate C (P_60) being the most significant. However, their high variable importance of projection (VIP) scores (>5) suggest these metabolites are potential diagnostic metabolites for for "dual" bioactivity against and The statistical models also showed the distinctiveness of metabolites produced by lichens and ELF, thus supporting our hypotheses of ELF functionality similar to plant endophytes.

摘要

使用代谢组学方法对三种地衣（(Stirt.) Zahlbr.、Zahlbr.和Stirt.）以及九种相关的内生地衣真菌（ELF）进行了评估。所有研究的地衣粗提物均对（最低抑菌浓度（MIC）：0.0625毫克/毫升）具有抗菌活性，但对未观察到抗菌活性，而ELF提取物被发现对（MIC：2.5毫克/毫升）和（MIC：5毫克/毫升）最具活性。对进行了分离并测试其对的抗菌活性。组分XvFr1至5对两种测试细菌均显示出生物活性。使用高分辨率液相色谱-质谱联用仪对选定的粗提物和组分进行代谢组学分析。多变量分析表明，生物活性组分XvFr1至3特有的五种次生代谢产物的存在，这些次生代谢产物被确定为具有抗菌活性的原因。这些代谢产物的-值处于显著性水平的边缘，其中甲基木糖酸C（P_60）最为显著。然而，它们较高的投影变量重要性（VIP）得分（>5）表明这些代谢产物是对和具有“双重”生物活性的潜在诊断代谢产物。统计模型还显示了地衣和ELF产生的代谢产物的独特性，从而支持了我们关于ELF功能类似于植物内生菌的假设。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/35ddfb2a1b5b/biology-10-00191-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/1eac03adcfd1/biology-10-00191-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/71c1acada107/biology-10-00191-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/951f8a822c60/biology-10-00191-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/4f95c10fb2fc/biology-10-00191-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/ab145588e213/biology-10-00191-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/35ddfb2a1b5b/biology-10-00191-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/1eac03adcfd1/biology-10-00191-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/71c1acada107/biology-10-00191-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/951f8a822c60/biology-10-00191-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/4f95c10fb2fc/biology-10-00191-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/ab145588e213/biology-10-00191-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e88/8000601/35ddfb2a1b5b/biology-10-00191-g006.jpg

相似文献

1
Biodiscovery of Potential Antibacterial Diagnostic Metabolites from the Endolichenic Fungus Using LC-MS-Based Metabolomics.基于液相色谱-质谱联用代谢组学从内生地衣真菌中发现潜在抗菌诊断代谢物
Biology (Basel). 2021 Mar 4;10(3):191. doi: 10.3390/biology10030191.
2
Isolation and characterization of bioactive metabolites from Xylaria psidii, an endophytic fungus of the medicinal plant Aegle marmelos and their role in mitochondrial dependent apoptosis against pancreatic cancer cells.从药用植物木苹果的内生真菌绿粘褶菌中分离并鉴定生物活性代谢产物及其在针对胰腺癌细胞的线粒体依赖性凋亡中的作用
Phytomedicine. 2016 Nov 15;23(12):1312-1320. doi: 10.1016/j.phymed.2016.07.004. Epub 2016 Jul 18.
3
Metabolome Comparison of Bioactive and Inactive Rhododendron Extracts and Identification of an Antibacterial Cannabinoid(s) from Rhododendron collettianum.活性与非活性杜鹃提取物的代谢组学比较及来自粘毛杜鹃的一种抗菌大麻素的鉴定
Phytochem Anal. 2017 Sep;28(5):454-464. doi: 10.1002/pca.2694. Epub 2017 Jun 13.
4
In vitro Antibacterial Activity and Secondary Metabolite Profiling of Endolichenic Fungi Isolated from Genus Parmotrema.从地梅属内生真菌中分离得到的内生真菌的体外抗菌活性和次生代谢产物分析。
Curr Microbiol. 2024 May 29;81(7):195. doi: 10.1007/s00284-024-03719-4.
5
Nature and bioactivities of endolichenic fungi in Pseudocyphellaria sp., Parmotrema sp. and Usnea sp. at Hakgala montane forest in Sri Lanka.斯里兰卡哈克加拉山地森林中假杯点衣属、黄髓衣属和松萝属地衣内生真菌的性质及生物活性
Lett Appl Microbiol. 2009 Feb;48(2):203-9. doi: 10.1111/j.1472-765X.2008.02512.x. Epub 2008 Jan 3.
6
Endolichenic fungus, Aspergillus quandricinctus of Usnea longissima inhibits quorum sensing and biofilm formation of Pseudomonas aeruginosa PAO1.长松萝内生真菌，纠缠青霉对铜绿假单胞菌 PAO1 群体感应和生物膜形成的抑制作用。
Microb Pathog. 2020 Mar;140:103933. doi: 10.1016/j.micpath.2019.103933. Epub 2019 Dec 17.
7
Molecular phylogeny and bioprospecting of Endolichenic Fungi (ELF) inhabiting in the lichens collected from a mangrove ecosystem in Sri Lanka.分子系统发育与生物勘探斯里兰卡红树林生态系统中内生真菌（ELF）
PLoS One. 2018 Aug 29;13(8):e0200711. doi: 10.1371/journal.pone.0200711. eCollection 2018.
8
Evaluation of the Antibacterial Activity of Crude Extracts Obtained From Cultivation of Native Endophytic Fungi Belonging to a Tropical Montane Rainforest in Colombia.对从哥伦比亚热带山地雨林中分离得到的本地内生真菌培养物粗提物抗菌活性的评估。
Front Microbiol. 2021 Sep 17;12:716523. doi: 10.3389/fmicb.2021.716523. eCollection 2021.
9
An acetonic extract and secondary metabolites from the endolichenic fungus sp. EL006872 exhibit immune checkpoint inhibitory activity in lung cancer cell.来自内生地衣真菌sp. EL006872的丙酮提取物和次生代谢产物在肺癌细胞中表现出免疫检查点抑制活性。
Front Pharmacol. 2022 Sep 8;13:986946. doi: 10.3389/fphar.2022.986946. eCollection 2022.
10
Eremoxylarins D-J, Antibacterial Eremophilane Sesquiterpenes Discovered from an Endolichenic Strain of .艾氏木香烷素D-J，从一种内生地衣菌株中发现的具有抗菌活性的艾里莫芬烷倍半萜类化合物
J Nat Prod. 2023 Apr 28;86(4):730-738. doi: 10.1021/acs.jnatprod.2c00881. Epub 2023 Mar 9.

引用本文的文献

1
Comparative Antagonistic Activities of Endolichenic Fungi Isolated from the Fruticose Lichens and .从枝状地衣中分离出的内生地衣真菌的比较拮抗活性 以及 。（原文此处不完整）
J Fungi (Basel). 2025 Apr 10;11(4):302. doi: 10.3390/jof11040302.
2
Metabolomic Approach in Anticancer Biomarker Discovery from Foliose Lichens.从叶状地衣中发现抗癌生物标志物的代谢组学方法。
Adv Pharm Bull. 2024 Dec 30;14(4):938-943. doi: 10.34172/apb.43220. Epub 2024 Dec 13.
3
Diversity and biological activities of endophytic fungi: insights from in-silico docking studies.

本文引用的文献

1
Endophytic and endolichenic fungal diversity in maritime Antarctica based on cultured material and their evolutionary position among .基于培养材料的南极海洋地区内生真菌和地衣内生真菌多样性及其在……中的进化位置
Fungal Syst Evol. 2018 Dec;2:263-272. doi: 10.3114/fuse.2018.02.07. Epub 2018 Aug 10.
2
Using MetaboAnalyst 4.0 for Comprehensive and Integrative Metabolomics Data Analysis.使用MetaboAnalyst 4.0进行全面综合的代谢组学数据分析。
Curr Protoc Bioinformatics. 2019 Dec;68(1):e86. doi: 10.1002/cpbi.86.
3
The potential of compounds isolated from Xylaria spp. as antifungal agents against anthracnose.
内生真菌的多样性与生物活性：基于计算机模拟对接研究的见解
Braz J Microbiol. 2025 Jun;56(2):797-815. doi: 10.1007/s42770-025-01616-x. Epub 2025 Feb 11.
4
Identification and determination of usnic acid and fatty acid from various lichen species in arequipa, Peru, as well as antibacterial and antioxidant capacity.秘鲁阿雷基帕不同地衣物种中美登木酸和脂肪酸的鉴定与测定，以及抗菌和抗氧化能力。
Heliyon. 2024 Oct 22;10(21):e39703. doi: 10.1016/j.heliyon.2024.e39703. eCollection 2024 Nov 15.
5
Investigation of Antimicrobial Compounds Produced by Endolichenic Fungi in Different Culture Media.不同培养基中内生地衣真菌产生的抗菌化合物的研究
Plant Pathol J. 2024 Oct;40(5):559-567. doi: 10.5423/PPJ.NT.06.2024.0087. Epub 2024 Oct 1.
6
A Close View of the Production of Bioactive Fungal Metabolites Mediated by Chromatin Modifiers.近距离观察染色质修饰物介导的生物活性真菌代谢产物的产生。
Molecules. 2024 Jul 27;29(15):3536. doi: 10.3390/molecules29153536.
7
Comparative Study on the Antimicrobial Activities and Metabolic Profiles of Five Species from the Philippines.菲律宾五种物种抗菌活性与代谢谱的比较研究
J Fungi (Basel). 2023 Nov 17;9(11):1117. doi: 10.3390/jof9111117.
8
Characterization and identification of antimicrobial compounds from endophytic Fusarium incarnatum isolated from Cymbidium orchids.从蝴蝶兰内生尖孢镰刀菌中分离得到的抗菌化合物的特性和鉴定。
Int Microbiol. 2024 Aug;27(4):977-992. doi: 10.1007/s10123-023-00442-1. Epub 2023 Nov 17.
9
Unveiling Chemical Interactions Between Plants and Fungi Using Metabolomics Approaches.利用代谢组学方法揭示植物和真菌之间的化学相互作用。
Adv Exp Med Biol. 2023;1439:1-20. doi: 10.1007/978-3-031-41741-2_1.
10
Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture of Species.基于非靶向和靶向液相色谱-串联质谱的物种体外培养代谢组学研究
J Fungi (Basel). 2022 Jan 6;8(1):55. doi: 10.3390/jof8010055.
从炭角菌属物种中分离出的化合物作为抗炭疽病真菌剂的潜力。
Braz J Microbiol. 2018 Oct-Dec;49(4):840-847. doi: 10.1016/j.bjm.2018.03.003. Epub 2018 Mar 31.
4
Secondary metabolism in the lichen symbiosis.共生地衣中的次生代谢。
Chem Soc Rev. 2018 Mar 5;47(5):1730-1760. doi: 10.1039/c7cs00431a.
5
New β-orcinol depsides from Hypotrachyna caraccensis, a lichen from the páramo ecosystem and their free radical scavenging activity.来自帕拉莫生态系统地衣卡拉卡Hypotrachyna caraccensis的新型β-苔黑酚缩酚酸及其自由基清除活性。
Nat Prod Res. 2018 Jun;32(12):1375-1382. doi: 10.1080/14786419.2017.1346639. Epub 2017 Jul 10.
6
Investigation of antioxidant, antimicrobial and toxicity activities of lichens from high altitude regions of Nepal.尼泊尔高海拔地区地衣的抗氧化、抗菌及毒性活性研究。
BMC Complement Altern Med. 2017 May 25;17(1):282. doi: 10.1186/s12906-017-1797-x.
7
Endolichenic Fungi: A Hidden Reservoir of Next Generation Biopharmaceuticals.内生真菌：下一代生物制药的隐藏宝库。
Trends Biotechnol. 2017 Sep;35(9):808-813. doi: 10.1016/j.tibtech.2017.03.003. Epub 2017 Mar 28.
8
Synthesis and anti-inflammatory activity of novel triazole hybrids of (+)-usnic acid, the major dibenzofuran metabolite of the lichen Usnea longissima.地衣长松萝主要二苯并呋喃代谢产物（+）-松萝酸新型三唑杂化物的合成及其抗炎活性
Mol Divers. 2017 May;21(2):273-282. doi: 10.1007/s11030-016-9716-5. Epub 2017 Jan 27.
9
Quinone derivatives isolated from the endolichenic fungus Phialocephala fortinii are Mdr1 modulators that combat azole resistance in Candida albicans.从内生真菌 Phialocephala fortinii 中分离得到的醌衍生物是 Mdr1 调节剂，可对抗白色念珠菌中的唑类耐药性。
Sci Rep. 2016 Sep 21;6:33687. doi: 10.1038/srep33687.
10
Comparative mass spectrometry-based metabolomics strategies for the investigation of microbial secondary metabolites.基于比较质谱的代谢组学策略用于微生物次级代谢产物的研究。
Nat Prod Rep. 2017 Jan 4;34(1):6-24. doi: 10.1039/c6np00048g.