物种水平的粪便微生物群分析是否是合适的临床生物标志物？一项针对病态肥胖受试者的初步研究。
Are Faecal Microbiota Analyses on Species-Level Suitable Clinical Biomarkers? A Pilot Study in Subjects with Morbid Obesity.
作者信息
Farup Per G, Maseng Maria G
机构信息
Department of Research, Innlandet Hospital Trust, N-2381 Brumunddal, Norway.
Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
出版信息
Microorganisms. 2021 Mar 23;9(3):664. doi: 10.3390/microorganisms9030664.
BACKGROUND
An abnormal faecal microbiota could be a causal factor for disease. This study evaluated a new method for faecal microbiota analysis in subjects with obesity and irritable bowel syndrome.
METHODS
The study had a matched case-control design. Forty-six subjects with morbid obesity (defined as BMI > 40 or >35 kg/m with obesity-related complications) of whom 23 had irritable bowel syndrome (IBS), were compared with 46 healthy volunteers. The faecal microbiota was analysed with Precision Microbiome Profiling (PMP™) which quantified 104 bacteria species. The primary aim was comparisons between the cases and controls.
RESULTS
Two men and 44 women with a mean age of 43.6 years were included in each of the groups; BMI in the groups was (mean and SD) 41.9 (3.5) and 22.5 (1.5) kg/m, respectively. Seventeen bacterial species showed statistically significant differences between the groups after adjusting for multiple testing. In a post hoc analysis, the sensitivity and specificity were 78%. Alpha diversity was lower in the group with obesity. In subjects with morbid obesity, no clinically significant differences were seen between subjects with and without IBS or from before to six months after bariatric surgery.
CONCLUSIONS
The results encourage further evaluation of the new microbiome profiling tool.
背景
粪便微生物群异常可能是疾病的一个致病因素。本研究评估了一种用于分析肥胖和肠易激综合征患者粪便微生物群的新方法。
方法
本研究采用匹配病例对照设计。将46例病态肥胖患者（定义为BMI>40或BMI>35kg/m²且伴有肥胖相关并发症）与46名健康志愿者进行比较，其中23例病态肥胖患者患有肠易激综合征（IBS）。采用精准微生物群分析（PMP™）对粪便微生物群进行分析，该方法可对104种细菌进行定量。主要目的是比较病例组和对照组。
结果
每组纳入2名男性和44名女性，平均年龄43.6岁；两组的BMI（均值和标准差）分别为41.9（3.5）和22.5（1.5）kg/m²。在进行多重检验校正后，17种细菌在两组之间存在统计学显著差异。在事后分析中，敏感性和特异性为78%。肥胖组的α多样性较低。在病态肥胖患者中，患有和未患有IBS的患者之间或在减肥手术前至术后6个月之间未观察到临床显著差异。
结论
这些结果鼓励对新的微生物群分析工具进行进一步评估。
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