Monteiro-Reis Sara, Carvalho-Maia Carina, Bart Genevieve, Vainio Seppo J, Pedro Juliana, Silva Eunice R, Sales Goreti, Henrique Rui, Jerónimo Carmen
Cancer Biology & Epigenetics Group, Research Center, Portuguese Oncology Institute of Porto (CI-IPOP), R. Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
Biobank, Department of Pathology, Portuguese Oncology Institute of Porto (IPOP), R. Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
Int J Mol Sci. 2021 Mar 23;22(6):3267. doi: 10.3390/ijms22063267.
Secreted extracellular vesicles (EVs) are heterogeneous cell-derived membranous granules which carry a large diversity of molecules and participate in intercellular communication by transferring these molecules to target cells by endocytosis. In the last decade, EVs' role in several pathological conditions, from etiology to disease progression or therapy evasion, has been consolidated, including in central nervous system (CNS)-related disorders. For this review, we performed a systematic search of original works published, reporting the presence of molecular components expressed in the CNS via EVs, which have been purified from plasma, serum or cerebrospinal fluid. Our aim is to provide a list of molecular EV components that have been identified from both nonpathological conditions and the most common CNS-related disorders. We discuss the methods used to isolate and enrich EVs from specific CNS-cells and the relevance of its components in each disease context.
分泌的细胞外囊泡（EVs）是异质性的细胞来源膜性颗粒，其携带多种分子，并通过内吞作用将这些分子转移至靶细胞，从而参与细胞间通讯。在过去十年中，EVs在从病因学、疾病进展到治疗逃逸等多种病理状况中的作用已得到确证，包括在中枢神经系统（CNS）相关疾病中。在本综述中，我们对已发表的原始研究进行了系统检索，这些研究报道了通过EVs在中枢神经系统中表达的分子成分的存在情况，这些EVs已从血浆、血清或脑脊液中纯化出来。我们的目的是提供一份已从非病理状况和最常见的中枢神经系统相关疾病中鉴定出的分子EV成分清单。我们讨论了从特定中枢神经系统细胞中分离和富集EVs的方法，以及其成分在每种疾病背景下的相关性。
