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DNA“定向”化学修饰的理论分析

Theoretical analysis of 'addressed' chemical modification of DNA.

作者信息

Perelroyzen M P, Vologodskii A V

机构信息

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences, USSR.

出版信息

Nucleic Acids Res. 1988 May 25;16(10):4693-704. doi: 10.1093/nar/16.10.4693.

DOI:10.1093/nar/16.10.4693
PMID:3380694
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336658/
Abstract

Chemical "addressed" modification of DNA involves treatment of single-stranded DNA with oligonucleotides complementary to certain target sequences in this DNA and bearing a groupings reactive towards DNA bases. The binding of oligonucleotides can occur both at completely (specific) and incompletely (nonspecific) complementary sites. We analyse the modification of a fragment that is flanked by two target sequences complementary to a given oligonucleotide address, contains no more such targets and has some randomly distributed sites for nonspecific binding. Conditions for the maximum ratio between specific and non-specific modification are determined. We find the probability of both target termini being specifically modified without any non-specific modification occurring within the fragment up to a given moment in time. Quantitative analysis is based on the use of known features of the specific and non-specific binding of an oligonucleotide to DNA sites. This analysis shows the possibility of specific cutting of DNA based on addressed modification.

摘要

DNA的化学“靶向”修饰涉及用与该DNA中某些靶序列互补且带有对DNA碱基具有反应性基团的寡核苷酸处理单链DNA。寡核苷酸的结合可发生在完全（特异性）和不完全（非特异性）互补位点。我们分析了一个片段的修饰情况，该片段两侧有两个与给定寡核苷酸靶标互补的靶序列，不再包含更多此类靶标，并且有一些随机分布的非特异性结合位点。确定了特异性修饰与非特异性修饰之间最大比例的条件。我们发现了在片段内直到给定时刻都没有任何非特异性修饰发生的情况下，两个靶末端都被特异性修饰的概率。定量分析基于寡核苷酸与DNA位点特异性和非特异性结合的已知特征。该分析表明了基于靶向修饰进行DNA特异性切割的可能性。

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