Mattiuzzo Elena, Faggian Alessia, Venerando Rina, Benetti Andrea, Belluzzi Elisa, Abatangelo Giovanni, Ruggieri Pietro, Brun Paola
Department of Molecular Medicine, Histology Unit, University of Padova, 35121 Padova, Italy.
Department of Molecular Medicine, University of Padova, 35121 Padova, Italy.
Pharmaceuticals (Basel). 2021 Mar 23;14(3):286. doi: 10.3390/ph14030286.
β-caryophyllene (BCP), a plant-derived sesquiterpene, has been reported to have anti-inflammatory and antioxidant effects. The purpose of this study is to evaluate the effects of BCP in combination with ascorbic acid (AA) and d-glucosamine (GlcN) against macrophage-mediated inflammation on in vitro primary human chondrocytes. Changes in cell viability, intracellular ROS generation, gene expression of pro-inflammatory mediators, metalloproteinases (MMPs), collagen type II and aggrecan were analyzed in primary human chondrocytes exposed to the conditioned medium (CM) of activated U937 monocytes and subsequently treated with BCP alone or in combination with AA and GlcN. The CM-induced chondrocyte cytotoxicity was reduced by the presence of low doses of BCP alone or in combination with AA and GlcN. The exposure of cells to CM significantly increased κ1 and expression, but when BCP was added to the inflamed cells, alone or in combination with AA and GlcN, gene transcription for all these molecules was restored to near baseline values. Moreover, chondrocytes increased the expression of and when stimulated with AA and GlcN alone or in combination with BCP. This study showed the synergistic anti-inflammatory and antioxidative effects of BCP, AA and GlcN at low doses on human chondrocyte cultures treated with the CM of activated U937 cells. Moreover, the combination of the three molecules was able to promote the expression of and . All together, these data could suggest that BCP, AA and GlcN exert a chondro-protective action.
β-石竹烯（BCP）是一种植物来源的倍半萜烯，据报道具有抗炎和抗氧化作用。本研究的目的是评估BCP与抗坏血酸（AA）和D-葡萄糖胺（GlcN）联合使用对体外原代人软骨细胞中巨噬细胞介导的炎症的影响。在暴露于活化的U937单核细胞的条件培养基（CM）并随后单独用BCP或与AA和GlcN联合处理的原代人软骨细胞中，分析细胞活力、细胞内活性氧生成、促炎介质、金属蛋白酶（MMPs）、II型胶原蛋白和聚集蛋白聚糖的基因表达变化。单独或与AA和GlcN联合使用低剂量的BCP可降低CM诱导的软骨细胞细胞毒性。细胞暴露于CM会显著增加κ1和表达，但当将BCP单独或与AA和GlcN联合添加到炎症细胞中时，所有这些分子的基因转录恢复到接近基线值。此外，当单独用AA和GlcN或与BCP联合刺激时，软骨细胞会增加和的表达。本研究表明，低剂量的BCP、AA和GlcN对用活化的U937细胞的CM处理的人软骨细胞培养物具有协同抗炎和抗氧化作用。此外，这三种分子的组合能够促进和的表达。总之，这些数据表明BCP、AA和GlcN发挥软骨保护作用。
