Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra 00233, Ghana.
Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana.
Med Sci (Basel). 2021 Mar 31;9(2):23. doi: 10.3390/medsci9020023.
Globally, the application of medicinal plants in the management of acute and chronic wounds can be considered a common occurrence in most traditional medicine practices. In view of this, many plants in the tropical and subtropical regions have been screened for their wound-healing activities. Consequently, plants having antimicrobial activity against multidrug-resistant (MD-R) pathogens can be considered great assets. Therefore, this study evaluated ethanolic and aqueous extracts of five medicinal plants (, , , , and ) for their antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds; Methods: This involved the aqueous and ethanolic extraction of the selected medicinal plants. Preliminary phytochemical constituents of the plants were examined. The agar well diffusion method was then used to determine the antibacterial activity of the leaves against reference strains ( ATCC 25922, ATCC 19430, ATCC 27853 and ATCC 25923, and a Methicillin-Resistant strain) as well as the MDR clinical isolates (, , and ) from the wounds; Results: The preliminary phytochemical analysis of the leaves showed the presence of saponins, phenolics, and reducing sugars in almost all the plants tested. All plant extracts were observed to have some antimicrobial activity against at least one reference strain. For the clinical MDR isolates, from this study showed highest inhibition to growth of all bacteria used. Activity of against was highest as compared to that of and . Similar observation was made for , and where the highest activity was observed against ; Conclusion: This study has mainly shown that , , , , and exhibits antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds. Also, these plants has bioactive phytochemical compounds with potential medicinal values for the treatment of numerous infections. Therefore, these plants may be helpful in the management of acute and chronic wounds, especially in traditional medicine practices.
在全球范围内，药用植物在治疗急性和慢性伤口方面的应用可以被认为是大多数传统医学实践中的常见现象。鉴于此，许多热带和亚热带地区的植物已经被筛选出具有伤口愈合活性。因此，具有抗多药耐药（MD-R）病原体抗菌活性的植物可以被视为宝贵的资源。因此，本研究评估了五种药用植物（、、、、和）的乙醇和水提取物对术后伤口分离的 MD-R 细菌病原体的抗菌活性；方法：这涉及到选定药用植物的水提和醇提。植物的初步植物化学成分进行了检查。然后使用琼脂孔扩散法来确定叶片对参考菌株（ATCC 25922、ATCC 19430、ATCC 27853 和 ATCC 25923 以及耐甲氧西林的一株）以及来自伤口的 MDR 临床分离株（、、和）的抗菌活性；结果：叶片的初步植物化学分析表明，几乎所有测试的植物都存在皂甙、酚类和还原糖。所有植物提取物都对至少一种参考菌株表现出一定的抗菌活性。对于本研究中的临床 MDR 分离株，来自 的抑制作用最强。与 和 相比， 对 的抑制作用最强。对于 、 和 也观察到类似的情况，其中对 的活性最高；结论：本研究主要表明，、、、和 对术后伤口分离的 MD-R 细菌病原体具有抗菌活性。此外，这些植物具有具有潜在药用价值的生物活性植物化学化合物，可用于治疗多种感染。因此，这些植物可能有助于急性和慢性伤口的管理，特别是在传统医学实践中。
