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使用透明质酸/海藻酸钠生物墨水的三维可打印水凝胶

Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink.

作者信息

Lee Su Jeong, Seok Ji Min, Lee Jun Hee, Lee Jaejong, Kim Wan Doo, Park Su A

机构信息

Medical Device Convergence Center, Konyang University Hospital, Daejeon 35365, Korea.

Department of Nature-Inspired System and Application, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Korea.

出版信息

Polymers (Basel). 2021 Mar 5;13(5):794. doi: 10.3390/polym13050794.

DOI:10.3390/polym13050794
PMID:33807639
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7961573/
Abstract

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (/). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% /), 1211 Pa∙s (HA, 10% /), and 1525 Pa∙s, (HA, 30% /) at a 0.1 s shear rate. Their structures exhibited no significant shrinkage after CaCl crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

摘要

生物墨水的特性已被广泛研究，用于组织工程应用的三维（3D）生物打印过程。在本研究中，我们开发了一种使用透明质酸（HA）和海藻酸钠（SA）合成生物墨水的方法，且不使用HA的化学交联剂至30%（/）。此外，我们评估了所得生物墨水的特性，以衡量它们在生物打印中的适用性，主要关注其粘度、可打印性和收缩特性。此外，使用活/死检测和WST-1对包裹细胞（NIH3T3成纤维细胞系）的生物墨水进行表征，以评估其生物相容性。结果表明，所有组的混合水凝胶在0.1 s剪切速率下均成功打印，粘度分别为883 Pa∙s（HA，0% /）、1211 Pa∙s（HA，10% /）和1525 Pa∙s（HA，30% /）。它们的结构在CaCl交联后没有明显收缩，并在培养期间保持完整。在第四天之后，HA/SA混合生物墨水中包裹细胞的相对增殖率比仅含SA的生物墨水高70%。这些结果表明，3D可打印的HA/SA水凝胶可用作组织工程应用的生物墨水。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/dd702484bb9b/polymers-13-00794-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/63ef0eaab5f4/polymers-13-00794-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/05e61a73c567/polymers-13-00794-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/0a7ddc758a55/polymers-13-00794-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/67bcb573b5e4/polymers-13-00794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/f4ccbabcac87/polymers-13-00794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/1d21f2450d9d/polymers-13-00794-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/dd702484bb9b/polymers-13-00794-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/63ef0eaab5f4/polymers-13-00794-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/05e61a73c567/polymers-13-00794-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/0a7ddc758a55/polymers-13-00794-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/67bcb573b5e4/polymers-13-00794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/f4ccbabcac87/polymers-13-00794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/1d21f2450d9d/polymers-13-00794-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c9/7961573/dd702484bb9b/polymers-13-00794-g007.jpg

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