Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari "Aldo Moro", Via Amendola 165/A, 70126, Bari, Italy.
Istituto per la Protezione Sostenibile delle Piante (IPSP) - CNR, UOS Bari, Via Amendola 122/D, 70126, Bari, Italy.
Sci Rep. 2020 Feb 13;10(1):2538. doi: 10.1038/s41598-020-59421-5.
Grafting of commercial tomato varieties and hybrids on the tomato ecotype Manduria resulted in high levels of tolerance to the infection of Sw5 resistance-breaking strains of tomato spotted wilt virus and of severe cucumber mosaic virus strains supporting hypervirulent satellite RNAs that co-determine stunting and necrotic phenotypes in tomato. To decipher the basis of such tolerance, here we used a RNAseq analysis to study the transcriptome profiles of the Manduria ecotype and of the susceptible variety UC82, and of their graft combinations, exposed or not to infection of the potato virus Y recombinant strain PVY-to. The analysis identified graft- and virus-responsive mRNAs differentially expressed in UC82 and Manduria, which led to an overall suitable level of tolerance to viral infection confirmed by the appearance of a recovery phenotype in Manduria and in all graft combinations. The transcriptome analysis suggested that graft wounding and viral infection had diverging effects on tomato transcriptome and that the Manduria ecotype was less responsive than the UC82 to both graft wounding and potyviral infection. We propose that the differential response to the two types of stress could account for the tolerance to viral infection observed in the Manduria ecotype as well as in the susceptible tomato variety UC82 self-grafted or grafted on the Manduria ecotype.
将商业番茄品种和杂种嫁接到番茄生态型曼都里亚上，导致对感染 Sw5 抗性突破株系的番茄斑点萎蔫病毒和严重的黄瓜花叶病毒株系的高度耐受性，这些株系支持超毒卫星 RNA，共同决定番茄的矮化和坏死表型。为了解释这种耐受性的基础，我们在这里使用 RNAseq 分析研究了曼都里亚生态型和易感品种 UC82 及其嫁接组合的转录组谱，这些组合暴露或不暴露于马铃薯 Y 重组株系 PVY-to 的感染。分析确定了在 UC82 和曼都里亚中差异表达的嫁接和病毒响应的 mRNAs，这导致了对病毒感染的总体适宜水平的耐受性，这在曼都里亚和所有嫁接组合中表现出恢复表型得到了证实。转录组分析表明，嫁接创伤和病毒感染对番茄转录组有不同的影响，曼都里亚生态型对嫁接创伤和马铃薯病毒感染的反应不如 UC82 强烈。我们提出，对这两种类型的应激的不同反应可以解释在曼都里亚生态型以及易感番茄品种 UC82 自身嫁接或嫁接到曼都里亚生态型上观察到的对病毒感染的耐受性。
