O'Conner Seth, Zheng Wenguang, Qi Mingsheng, Kandel Yuba, Fuller Robert, Whitham Steven A, Li Ling
Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA.
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.
Int J Mol Sci. 2021 Mar 30;22(7):3586. doi: 10.3390/ijms22073586.
The NF-Y gene family is a highly conserved set of transcription factors. The functional transcription factor complex is made up of a trimer between NF-YA, NF-YB, and NF-YC proteins. While mammals typically have one gene for each subunit, plants often have multigene families for each subunit which contributes to a wide variety of combinations and functions. Soybean plants with an overexpression of a particular NF-YC isoform (Glyma.04g196200) in soybean cultivar Williams 82, had a lower amount of starch in its leaves, a higher amount of protein in its seeds, and increased broad disease resistance for bacterial, viral, and fungal infections in the field, similar to the effects of overexpression of its isoform (Glyma.06g169600). Interestingly, (overexpression) plants also filled pods and senesced earlier, a novel trait not found in plants. No yield difference was observed in compared with the wild-type control. Sequence alignment of GmNF-YC4-2, GmNF-YC4-1 and AtNF-YC1 indicated that faster maturation may be a result of minor sequence differences in the terminal ends of the protein compared to the closely related isoforms.
NF-Y基因家族是一组高度保守的转录因子。功能性转录因子复合物由NF-YA、NF-YB和NF-YC蛋白三聚体组成。虽然哺乳动物的每个亚基通常只有一个基因，但植物的每个亚基往往有多个基因家族，这导致了多种组合和功能。在大豆品种Williams 82中过表达特定NF-YC亚型（Glyma.04g196200）的大豆植株，其叶片中的淀粉含量较低，种子中的蛋白质含量较高，并且在田间对细菌、病毒和真菌感染的广谱抗病性增强，类似于其亚型（Glyma.06g169600）过表达的效果。有趣的是，（过表达）植株的豆荚充实和衰老也更早，这是在 植株中未发现的新性状。与野生型对照相比，未观察到产量差异。GmNF-YC4-2、GmNF-YC4-1和AtNF-YC1的序列比对表明，与密切相关的亚型相比，更快成熟可能是蛋白质末端微小序列差异的结果。
