Zhang Mingyan, Ma Wuchao, Wang Chao, Yang Ximing, Lou Yuhang, Xia Xinxiu, Xu Hongyan
Department of Food Science and Engineering, Agricultural College, Yanbian University, Yanji 133000, China.
Department of Food Science and Engineering, Fusion College, Yanbian University, Yanji 133000, China.
Molecules. 2021 Mar 30;26(7):1950. doi: 10.3390/molecules26071950.
nut-coated film is a kind of by-product of nut processing, which has been shown to contain flavonoids, polyphenols, and other substances that can be used to produce natural antioxidant extracts. In this study, response surface methodology (RSM) was used to optimize the extraction process of flavonoids of nut-coated film (PNF), and macroporous resin HPD600 was used to purify PNF (P-PNF). Its antioxidant activity was examined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging capacity, oxygen free radical absorption capacity (ORAC), total oxygen radical capture (TRAP), and iron ion reduction capacity. Under the ideal extraction conditions comprising a cellulase dosage of 90 U/g, a material/liquid ratio of 1:20 (g/mL), and an extraction time of 2 h, the PNF yield was 3.37%. Purification conditions were sample concentration of 2.0 mg/mL, pH of 5, water washing volume of 3 bed volume (BV), eluent ethanol concentration of 50%, and volume of 2 BV. The P-PNF recovery was 84.32%, and purity increased from 33.80% to 61.70%. Additionally, P-PNF showed increased antioxidant activity compared to PNF. Cumulatively, this study obtained the optimal values for the process parameters in order to achieve the maximum rates of extraction of PNF for economically optimal production at an industrial scale.
坚果包衣膜是坚果加工的一种副产品，已被证明含有黄酮类化合物、多酚类物质以及其他可用于生产天然抗氧化提取物的物质。在本研究中，采用响应面法（RSM）优化坚果包衣膜（PNF）中黄酮类化合物的提取工艺，并使用大孔树脂HPD600对PNF进行纯化（P-PNF）。通过1,1-二苯基-2-苦基肼（DPPH）自由基清除能力、氧自由基吸收能力（ORAC）、总氧自由基捕获能力（TRAP）和铁离子还原能力来检测其抗氧化活性。在纤维素酶用量为90 U/g、料液比为1:20（g/mL）、提取时间为2 h的理想提取条件下，PNF得率为3.37%。纯化条件为样品浓度2.0 mg/mL、pH值为5、水洗体积3倍床体积（BV）、洗脱剂乙醇浓度50%、体积2 BV。P-PNF回收率为84.32%，纯度从33.80%提高到61.70%。此外，与PNF相比，P-PNF的抗氧化活性有所提高。总的来说，本研究获得了工艺参数的最佳值，以便在工业规模上实现经济最优生产时PNF的最大提取率。
