Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, 37 Chelmonskiego Street, 51-630 Wroclaw, Poland.
Faculty of Chemistry, University of Wroclaw, 14 F. Joliot-Curie Street, 50-383 Wroclaw, Poland.
Biomolecules. 2021 Mar 30;11(4):519. doi: 10.3390/biom11040519.
Biosurfactants represent a structurally diverse group of secondary metabolites produced by bacteria, yeasts, and filamentous fungi. Their character is often associated with numerous additional properties. The observation of fungi of various species used as a source of bioinhibitors against pathogenic plants fungi focuses attention to the often quite specific behavior of preparations in contact with, among others, plant leaves, dependent on strain. Thus, an evaluation of the selected strains belonging to the species: , and was conducted towards their capability of the extracellular secretion of surfactants, with a simultaneous attempt to pre-determine their chemical nature. Two mineral-organic media were used for this purpose, and the culture fluid was extensively tested using a variety of methods. A decrease in surface tension was observed in culture fluid of each tested strain, especially HL and C1. The results strongly depended on medium composition, of which Saunders 1 and MGP 1 were most beneficial. The secreted compounds were further analyzed to pre-determine their chemical nature using IR, GC, and NMR. In the case of most efficient biosurfactant producers, a lipopeptide structure of the surfactants was concluded.
生物表面活性剂是由细菌、酵母和丝状真菌产生的结构多样的次生代谢产物。它们的特性通常与许多其他特性相关联。观察到各种真菌用作生物抑制剂的来源，以对抗致病植物真菌，这引起了人们对制剂与植物叶片接触时的行为的关注，这种行为往往取决于菌株的不同。因此，对属于以下物种的选定菌株进行了评估： ， 和 ，以确定它们细胞外分泌表面活性剂的能力，并同时尝试预先确定它们的化学性质。为此目的使用了两种矿物有机培养基，并使用多种方法对培养液进行了广泛测试。在每种测试菌株的培养液中都观察到表面张力降低，尤其是 HL 和 C1。结果强烈依赖于培养基的组成，其中 Saunders 1 和 MGP 1 最有利。使用 IR、GC 和 NMR 进一步分析分泌的化合物以预先确定其化学性质。对于最有效的生物表面活性剂产生菌，得出了表面活性剂的脂肽结构。
