Rowińska Ilona, Szyperska-Ślaska Adrianna, Zariczny Piotr, Pasławski Robert, Kramkowski Karol, Kowalczyk Paweł
Medical and Social Center for Vocational and Continuing Education in Toruń, St. Jana 1/3, 87-100 Toruń, Poland.
Toruń City Hall, Business Support Center in Toruń, ul. Marii Konopnickiej 13, 87-100 Toruń, Poland.
Materials (Basel). 2021 Mar 12;14(6):1372. doi: 10.3390/ma14061372.
The diet is related to the diversity of bacteria in the oral cavity, and the less diverse microbiota of the oral cavity may favor the growth of pathogenic bacteria of all bacterial complexes. Literature data indicate that disturbances in the balance of the bacterial flora of the oral cavity seem to contribute to both oral diseases, including periodontitis, and systemic diseases. If left untreated, periodontitis can damage the gums and alveolar bones. Improper modern eating habits have an impact on the oral microbiome and the gut microbiome, which increase the risk of several chronic diseases, including inflammatory bowel disease, obesity, type 2 diabetes, cardiovascular disease and cancer. The subject of our consideration is the influence of the traditional diet on the formation of oxidative stress and inflammation caused by bacterial biofilm in the oral cavity. Through dental, biomedical and laboratory studies, we wanted to investigate the effect of individual nutrients contained in specific diets on the induction of oxidative stress inducing inflammation of the soft tissues in the oral cavity in the presence of residual supra- and subgingival biofilm. In our research we used different types of diets marked as W, T, B, F and noninvasively collected biological material in the form of bacterial inoculum from volunteers. The analyzed material was grown on complete and selective media against specific strains of all bacterial complexes. Additionally, the zones of growth inhibition were analyzed based on the disc diffusion method. The research was supplemented with dental and periodontological indicators. The research was supplemented by the application of molecular biology methods related to bacterial DNA isolation, PCR reactions and sequencing. Such selected methods constitute an ideal screening test for the analysis of oral bacterial microbiota. The obtained results suggest that certain types of diet can be an effective prophylaxis in the treatment of civilization diseases such as inflammation of the oral cavity along with periodontal tissues and gingival pockets.
饮食与口腔细菌的多样性有关，口腔中微生物群多样性较低可能有利于所有细菌复合体中病原菌的生长。文献数据表明，口腔细菌菌群平衡的紊乱似乎会导致口腔疾病（包括牙周炎）和全身性疾病。如果不进行治疗，牙周炎会损害牙龈和牙槽骨。现代不当的饮食习惯会对口腔微生物群和肠道微生物群产生影响，从而增加包括炎症性肠病、肥胖症、2型糖尿病、心血管疾病和癌症在内的几种慢性疾病的风险。我们研究的主题是传统饮食对口腔中细菌生物膜引起的氧化应激和炎症形成的影响。通过牙科、生物医学和实验室研究，我们想调查特定饮食中所含的个体营养素在存在龈上和龈下残余生物膜的情况下对诱导口腔软组织炎症的氧化应激的影响。在我们的研究中，我们使用了标记为W、T、B、F的不同类型饮食，并以志愿者细菌接种物的形式非侵入性收集生物材料。将分析的材料在针对所有细菌复合体特定菌株的完全培养基和选择性培养基上培养。此外，基于纸片扩散法分析生长抑制区。该研究还补充了牙科和牙周病学指标。通过应用与细菌DNA分离、PCR反应和测序相关的分子生物学方法对研究进行了补充。这些选定的方法构成了分析口腔细菌微生物群的理想筛选试验。所得结果表明，某些类型的饮食可以作为一种有效的预防措施，用于治疗诸如口腔炎症以及牙周组织和牙龈袋炎症等文明病。
