基于免疫细胞治疗中用于物理基因递送的信使核糖核酸电穿孔的来龙去脉
The Ins and Outs of Messenger RNA Electroporation for Physical Gene Delivery in Immune Cell-Based Therapy.
作者信息
Campillo-Davo Diana, De Laere Maxime, Roex Gils, Versteven Maarten, Flumens Donovan, Berneman Zwi N, Van Tendeloo Viggo F I, Anguille Sébastien, Lion Eva
机构信息
Tumor Immunology Group, Laboratory of Experimental Hematology, Faculty of Medicine and Health Sciences, Vaccine & Infectious Disease Institute (VAXINFECTIO), University of Antwerp, 2610 Wilrijk, Belgium.
Center for Cell Therapy & Regenerative Medicine, Antwerp University Hospital, 2650 Edegem, Belgium.
出版信息
Pharmaceutics. 2021 Mar 16;13(3):396. doi: 10.3390/pharmaceutics13030396.
Messenger RNA (mRNA) electroporation is a powerful tool for transient genetic modification of cells. This non-viral method of genetic engineering has been widely used in immunotherapy. Electroporation allows fine-tuning of transfection protocols for each cell type as well as introduction of multiple protein-coding mRNAs at once. As a pioneering group in mRNA electroporation, in this review, we provide an expert overview of the ins and outs of mRNA electroporation, discussing the different parameters involved in mRNA electroporation as well as the production of research-grade and production and application of clinical-grade mRNA for gene transfer in the context of cell-based immunotherapies.
信使核糖核酸（mRNA）电穿孔是一种用于细胞瞬时基因改造的强大工具。这种非病毒基因工程方法已在免疫治疗中广泛应用。电穿孔能够针对每种细胞类型微调转染方案，还能一次性导入多种蛋白质编码mRNA。作为mRNA电穿孔领域的先驱团队，在本综述中，我们对mRNA电穿孔的来龙去脉进行了专业概述，讨论了mRNA电穿孔涉及的不同参数，以及在基于细胞的免疫治疗背景下用于基因转移的研究级和临床级mRNA的生产及应用。
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