Wang Jing, Huang Xinyue, Xie Jiali, Han Yunwei, Huang Yuanshuai, Zhang Hongwei
Department of Blood Transfusion, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
Southwest Medical University, Luzhou, Sichuan, China.
Clin Chim Acta. 2021 Jul;518:142-150. doi: 10.1016/j.cca.2021.03.026. Epub 2021 Mar 31.
Exosomes, a subtype of extracellular vesicle secreted by cells, have been a subject of intense research interest. Unfortunately, a simple and reliable method to separate exosomes has yet to be developed. As can be expected, the lack of a standardized method for extraction and purification has contributed to suboptimal inter-laboratory correlation and difficulty in comparison studies. Traditional techniques such as centrifugation, immunoaffinity and size exclusion chromatography, suffer from low purity and tend to be labor intensive thus making their use limited. To mitigate these drawbacks, an integrated biosensor-based exosome separation and detection has recently been developed. In this review, we examine five biosensors that use a variety of detection technology (colorimetric, fluorescent, surface plasmon resonance, surface-enhanced Raman scattering and electrochemical) and propose thoughts on standardization of exosomal analysis.
外泌体是细胞分泌的细胞外囊泡的一种亚型，一直是研究的热点。不幸的是，尚未开发出一种简单可靠的外泌体分离方法。可以预料，缺乏标准化的提取和纯化方法导致实验室间的相关性欠佳，且比较研究存在困难。传统技术如离心、免疫亲和和尺寸排阻色谱法，存在纯度低且往往劳动强度大的问题，因此其应用受到限制。为了减轻这些缺点，最近开发了一种基于集成生物传感器的外泌体分离和检测方法。在这篇综述中，我们研究了五种使用各种检测技术（比色法、荧光法、表面等离子体共振、表面增强拉曼散射和电化学）的生物传感器，并对外泌体分析的标准化提出了看法。
