Department of Microbiology, University of North Bengal, Darjeeling, 734013, West Bengal, India.
Arch Microbiol. 2021 Aug;203(6):3135-3146. doi: 10.1007/s00203-021-02278-7. Epub 2021 Apr 4.
The present study is the first of its kind which is focused on Tsomgo lake, a high-altitude lake, located in the Eastern Himalayas of Sikkim. To get a major insight into the bacterial diversity, the shotgun sequencing was carried out in Illumina platform. Our results showed that both the samples TLSS1 (soil) and TLSW1 (water), had Proteobacteria as the most abundant taxa. Cluster of Orthologous group (COG) functional category of TLSS1 has 1,46,965 predicted functions. Cluster of Orthologous Group (COG) functional category of TLSW1 has 1,34,773 predicted functions. Kyoto Encyclopedia of Gene and Genomes (KEGG) functional category of TLSS1 has 1,76,825 predicted functions, most of the sequence fall in metabolism followed by Environmental information processing function. (KEGG) functional category of TLSW1 has 1,62,696 predicted functions and it follows the same pattern as TLSS1. Our studies also provide insight into the presence of distribution of different carbohydrate-active enzymes (CAZymes) present in Tsomgo lake. We have found that in case of both the samples TLSW1 and TLSS1, GlycosylTransferases were active followed by GlycosylHydrolase. The result found, represents for the first time very important findings related to the microbial diversity and the abundance of CAZymes in Tsomgo lake one of the pristine high-altitude lakes in Sikkim.
本研究首次聚焦于东喜马拉雅山锡金邦的高海拔湖泊——错高湖。为深入了解细菌多样性，我们在 Illumina 平台上进行了鸟枪法测序。结果表明，土壤样本 TLSS1 和湖水样本 TLSW1 中最丰富的菌群均为变形菌门。TLSS1 的基因集（COG）功能分类有 146965 个预测功能，TLSW1 有 134773 个预测功能。TLSS1 的京都基因与基因组百科全书（KEGG）功能分类有 176825 个预测功能，大部分序列都属于代谢功能，其次是环境信息处理功能。TLSW1 的 KEGG 功能分类有 162696 个预测功能，与 TLSS1 类似。本研究还深入了解了错高湖中不同碳水化合物活性酶（CAZymes）的分布情况。结果表明，在两个样本中，糖基转移酶最为活跃，其次是糖苷水解酶。本研究首次发现，错高湖作为锡金邦原始高海拔湖泊之一，其微生物多样性和 CAZymes 丰度具有重要意义。
