Faculty of Dentistry, Thammasat University.
Faculty of Dentistry, Chulalongkorn University.
Dent Mater J. 2021 Jul 31;40(4):934-941. doi: 10.4012/dmj.2020-307. Epub 2021 Apr 3.
Effects of histatin-derived peptides immobilization by tresyl chloride-activation technique for MC3T3-E1 cellular responses on titanium (Ti) were evaluated. MC3T3-E1 were cultured on sandblasted and acid-etched Ti disks immobilized with histatin-derived peptides, including histatin-1, JH8194, and mixed histatin-1 with JH8194. Surface topography and cellular morphology were examined using a scanning electron microscope. Elemental composition and conformational peptides on Ti surface were examined using energy dispersive X-ray and fourier transform infrared spectroscopy, respectively. Cellular adhesion, proliferation, osteogenesis-related genes, and alkaline phosphatase activity were evaluated. The results showed that peptides were successfully immobilized on Ti surface. Cell attachments on histatin-1 and mixed peptides coated groups are higher than control. Histatin-1 achieved the significantly highest cellular proliferation. Histatin-derived peptides improved the osteogenesis related-gene expression and alkaline phosphatase activity (p<0.05). This study suggested that histatin-1 immobilization by tresyl chloride-activation technique enhanced cellular responses and might be able to promote cellular activities around the dental implants.
采用三氯乙酰化激活技术固定组氨酸衍生肽对 MC3T3-E1 细胞在钛（Ti）表面上的细胞反应的影响进行了评估。将 MC3T3-E1 细胞培养在固定有组氨酸衍生肽的喷砂酸蚀 Ti 盘上，组氨酸衍生肽包括组氨酸-1、JH8194 和混合的组氨酸-1 和 JH8194。使用扫描电子显微镜检查表面形貌和细胞形态。使用能量色散 X 射线和傅里叶变换红外光谱分别检查 Ti 表面的元素组成和构象肽。评估细胞黏附、增殖、成骨相关基因和碱性磷酸酶活性。结果表明，肽已成功固定在 Ti 表面上。组氨酸-1 和混合肽涂层组的细胞附着高于对照。组氨酸-1 实现了最高的细胞增殖。组氨酸衍生肽改善了成骨相关基因的表达和碱性磷酸酶活性（p<0.05）。本研究表明，三氯乙酰化激活技术固定组氨酸-1 增强了细胞反应，可能能够促进牙种植体周围的细胞活性。
