Laboratory of Organic Chemistry, Natural Substances Team (LR17-ES08), Faculty of Sciences of Sfax, University of Sfax, PB "1171", PC "3000", Sfax, Tunisia.
Laboratory of Bioresources: Integrative Biology and Exploiting, Higher Institute of Biotechnology of Monastir, University of Monastir, Tunisia.
J Diabetes Res. 2021 Mar 17;2021:6659415. doi: 10.1155/2021/6659415. eCollection 2021.
Methanol and methanol/water extracts of olive stones and seeds from var. meski were analyzed by reversed-phase high-performance liquid chromatography (HPLC) with diode array detection and mass spectrometry (LC-MS/MS). A total of 28 metabolites were identified; among them are hydroxycinnamic acid derivatives, phenolic alcohols, flavonoids and flavonoid glucosides, secoiridoids, and terpenes. All the extracts were screened for the inhibitory effect of key enzymes related to diabetes and obesity, such as -amylase and lipase. An study revealed that meski stone ethanol (MSE) and methanol (MSM) extracts and meski seed ethanol (MSE1) and methanol (MSM1) extracts exert an inhibitory action against lipase and -amylase. The most potent activity was observed in the StM extract with IC equal to 0.19 mg/ml against DPPH oxidation, 1.04 mg/ml against -amylase, and 2.13 mg/ml against lipase. In HFFD rats, the findings indicated that the increase of body weight, LDL, TC, and glucose levels and then the decrease in HDL-C were significantly suppressed in the MSM-treated group than those in HFFD rats. Moreover, the MSM extract exhibited a prominent selective inhibitory effect against intestinal lipase and -amylase activities. The MSM extract was also able to protect the liver-kidney functions efficiently, which was evidenced by biochemicals and histological studies.
采用反相高效液相色谱法（HPLC）与二极管阵列检测和质谱法（LC-MS/MS）对来自 var. meski 的橄榄石和种子的甲醇和甲醇/水提取物进行了分析。共鉴定出 28 种代谢产物；其中包括羟基肉桂酸衍生物、酚类醇、类黄酮和类黄酮糖苷、裂环环烯醚萜和萜类化合物。所有提取物均针对与糖尿病和肥胖相关的关键酶（如α-淀粉酶和脂肪酶）的抑制作用进行了筛选。一项研究表明，meski 石乙醇（MSE）和甲醇（MSM）提取物和 meski 种子乙醇（MSE1）和甲醇（MSM1）提取物对脂肪酶和α-淀粉酶具有抑制作用。StM 提取物的活性最强，其对 DPPH 氧化的 IC 50 值为 0.19mg/ml，对α-淀粉酶的抑制率为 1.04mg/ml，对脂肪酶的抑制率为 2.13mg/ml。在 HFFD 大鼠中，研究结果表明，与 HFFD 大鼠相比，MSM 处理组大鼠体重、LDL、TC 和血糖水平的升高以及 HDL-C 的降低均受到显著抑制。此外，MSM 提取物对肠道脂肪酶和α-淀粉酶活性表现出显著的选择性抑制作用。MSM 提取物还能够有效地保护肝肾功能，这可以从生物化学和组织学研究中得到证明。
