Smith I, Cromie R, Stainsby K
School of Pathology, Middlesex Hospital Medical School, London, United Kingdom.
Anal Biochem. 1988 Mar;169(2):370-1. doi: 10.1016/0003-2697(88)90297-7.
Indicator dyes have been incorporated in the stacking gel monomer used in acrylamide gels to render readily visible and delineate the sample wells. Some dyes, such as bromphenol blue, migrate during the electrophoresis, whereas others, such as the ortho-unsubstituted phenol red, become chemically bound to the polymerized gel. The method can be used for agarose gels.
指示染料已被添加到用于丙烯酰胺凝胶的堆积凝胶单体中，以使样品孔易于看见并勾勒出其轮廓。一些染料，如溴酚蓝，在电泳过程中会迁移，而其他一些染料，如邻位未取代的酚红，则会与聚合凝胶发生化学结合。该方法可用于琼脂糖凝胶。
