McLeod M K, Thompson N W, Hudson J L, Gaglio J A, Lloyd R V, Harness J K, Nishiyama R, Cheung P S
Department of Surgery, University of Michigan Medical Center, Ann Arbor.
Arch Surg. 1988 Jul;123(7):849-54. doi: 10.1001/archsurg.1988.01400310063010.
Nuclear DNA content and nuclear DNA ploidy were measured in 36 Hürthle cell neoplasms (HCNs) to better define their potential roles in predicting the clinical behavior of these lesions. An EPICS V flow cytometer (Coulter Electronics, Hialeah, Fla) was used. Measurements were taken from paraffin-embedded tissue. Isolated nuclei were stained with propidium iodide. The study was conducted in a blinded fashion. Observed NDC and PDY patterns were classified as diploid, aneuploid, or suspicious. Twenty-nine lesions (81%) were diploid and seven (19%) were aneuploid. Twelve (33%) HCNs were malignant, 23 (64%) were benign, and one (3%) was indeterminate. Eight (67%) of 12 malignant HCNs were diploid and four (33%) of 12 were aneuploid. In comparison, 20 (87%) of 23 benign lesions were diploid and three (13%) of 23 were aneuploid. The indeterminate neoplasm was diploid. There were three deaths in this group of patients; all three had aneuploid neoplasms, and all had locally recurrent disease with distant metastases. There was a significant cross correlation between histologic features and DNA content with regard to outcome. These preliminary data suggest that NDC and PDY are not helpful in distinguishing histologically benign from malignant HCNs; however, they may be useful in determining prognosis.
对36例许特耳细胞肿瘤（HCNs）进行核DNA含量和核DNA倍体检测，以更好地明确其在预测这些病变临床行为中的潜在作用。使用EPICS V流式细胞仪（库尔特电子公司，佛罗里达州海厄利亚）。测量取自石蜡包埋组织。分离的细胞核用碘化丙啶染色。该研究采用盲法进行。观察到的核DNA含量（NDC）和倍体（PDY）模式分为二倍体、非整倍体或可疑。29个病变（81%）为二倍体，7个（19%）为非整倍体。12个（33%）HCNs为恶性，23个（64%）为良性，1个（3%）为不确定。12个恶性HCNs中有8个（67%）为二倍体，12个中有4个（33%）为非整倍体。相比之下，23个良性病变中有20个（87%）为二倍体，23个中有3个（13%）为非整倍体。不确定肿瘤为二倍体。该组患者中有3例死亡；所有3例均有非整倍体肿瘤，且均有局部复发伴远处转移。就结果而言，组织学特征与DNA含量之间存在显著的交叉相关性。这些初步数据表明，NDC和PDY在区分组织学上良性和恶性HCNs方面并无帮助；然而，它们可能有助于确定预后。
