Rainwater L M, Farrow G M, Hay I D, Lieber M M
Br J Cancer. 1986 Jun;53(6):799-804. doi: 10.1038/bjc.1986.135.
Nuclear DNA ploidy studies were performed by flow cytometry on extracted nuclei from 12 oncocytic tumours of the salivary gland, 65 oncocytic tumours of the kidney, and 37 oncocytic tumours of the thyroid gland from the pathology archives of the Mayo Clinic. In order to provide an interesting clinical spectrum, three different classes of well-differentiated oncocytic tumours were selected for examination. Salivary gland oncocytic tumours were chosen for their generally benign behaviour. Oncocytic thyroid cancers exhibiting malignant potential because of local invasion, were thought to represent the opposite extreme of aggressiveness. Renal oncocytic tumours were known to demonstrate an intermediate degree of malignancy. All of the oncocytic salivary gland tumours showed a 'normal' DNA histogram and had a benign clinical course. For the oncocytic tumours of the kidney, 45% of DNA histograms were normal, 40% exhibited a significant increase in the DNA tetraploid/polyploid (4C) peak, and 15% showed a DNA aneuploid peak. Three patients with a DNA tetraploid pattern developed tumour metastasis and two have died from metastatic renal cancer. Among the oncocytic thyroid cancers, 27% were normal, 22% exhibited an increased DNA tetraploid peak, and 51% had a distinct DNA aneuploid peak. None of the thyroid tumour patients with a normal DNA pattern or with an increased DNA tetraploid peak died as a result of thyroid malignancy. In contrast, 58% of patients whose thyroid tumours showed a DNA aneuploid peak subsequently died from thyroid cancer.
通过流式细胞术对从梅奥诊所病理档案中提取的细胞核进行了核DNA倍性研究，这些细胞核来自12例涎腺嗜酸细胞瘤、65例肾嗜酸细胞瘤和37例甲状腺嗜酸细胞瘤。为了提供一个有趣的临床谱，选择了三种不同类型的高分化嗜酸细胞瘤进行检查。选择涎腺嗜酸细胞瘤是因为它们通常具有良性行为。因局部侵袭而具有恶性潜能的嗜酸细胞性甲状腺癌被认为代表了侵袭性的相反极端。肾嗜酸细胞瘤已知表现出中等程度的恶性。所有涎腺嗜酸细胞瘤均显示“正常”的DNA直方图，且临床过程为良性。对于肾嗜酸细胞瘤，45%的DNA直方图正常，40%显示DNA四倍体/多倍体（4C）峰显著增加，15%显示DNA非整倍体峰。三名具有DNA四倍体模式的患者发生了肿瘤转移，两名患者死于转移性肾癌。在嗜酸细胞性甲状腺癌中，27%为正常，22%显示DNA四倍体峰增加，51%有明显的DNA非整倍体峰。DNA模式正常或DNA四倍体峰增加的甲状腺肿瘤患者均未因甲状腺恶性肿瘤死亡。相比之下，甲状腺肿瘤显示DNA非整倍体峰的患者中，58%随后死于甲状腺癌。
