Celik Cagla, Can Sezgin Gulten, Kocabas Umut Gorkem, Gursoy Sebnem, Ildiz Nilay, Tan Weihong, Ocsoy Ismail
Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey.
Pharmacy Services Program, Vocational School of Health Services, Hitit University, Corum 19000, Turkey.
Anal Chem. 2021 Apr 20;93(15):6246-6253. doi: 10.1021/acs.analchem.1c00663. Epub 2021 Apr 7.
Several different diagnostic tests have been reported for rapid, sensitive, and economical detection of bacterial pathogens, but most lack widespread and practical use in the clinic. In this study, we used anthocyanins from red cabbage () as a natural pH indicator and, for the first time, incorporated this agent into a simple, rapid, and economical colorimetric strategy for the detection of () (RCE@test). We prepared two sets of RCE@test solutions (test 1 is purple, and test 2 is blue) in different forms, including liquid, adsorbed filter paper, and agar, and investigated the performance of each RCE@test as a function of the test volume, concentration, and reaction time. To elucidate the effect of the pathophysiological environment on these RCE@tests, in an artificial gastric fluid was also detected. The 10 and 1 CFU/mL suspensions were detected in 15 min and 3 h, respectively, and the limit of detection was determined down to 1 CFU/mL. We experimentally demonstrated the advantages of the RCE@test for detection of by comparing it to a commercially available rapid urease test, the "CLO test (-like organism test)". In addition to colorimetric detection by the naked eyes, RGB (Red Green Blue) and Delta-E analysis in image-processing software was run to quantitatively monitor changes of color in the RCE@test using a smartphone application. Finally, we propose that this test provides simple, effective, rapid, and inexpensive detection and that it can be easily implemented for clinical use.
已有多种不同的诊断测试方法用于快速、灵敏且经济地检测细菌病原体，但大多数方法在临床中缺乏广泛且实际的应用。在本研究中，我们使用红甘蓝中的花青素作为天然pH指示剂，并首次将该试剂纳入一种简单、快速且经济的比色策略，用于检测幽门螺杆菌（RCE@test）。我们制备了两种不同形式的RCE@test溶液（测试1为紫色，测试2为蓝色），包括液体、吸附滤纸和琼脂，并研究了每种RCE@test作为测试体积、幽门螺杆菌浓度和反应时间函数的性能。为了阐明病理生理环境对这些RCE@test的影响，还检测了人工胃液中的幽门螺杆菌。分别在15分钟和3小时内检测到10和1 CFU/mL的幽门螺杆菌悬液，检测限低至1 CFU/mL。通过将RCE@test与市售快速尿素酶测试“CLO测试（类幽门螺杆菌测试）”进行比较，我们通过实验证明了RCE@test在检测幽门螺杆菌方面的优势。除了肉眼比色检测外，还使用图像处理软件进行RGB（红、绿、蓝）和Delta-E分析，以通过智能手机应用程序定量监测RCE@test中的颜色变化。最后，我们提出该测试提供了简单、有效、快速且廉价的检测方法，并且可以很容易地应用于临床。
