Jamia Hamdard, School of Pharmaceutical Education & Research, Department of Pharmacognosy and Phytochemistry, New Delhi 110062, India.
Taif University, College of Pharmacy, Department of Pharmaceutical Chemistry, P.O. Box 11099, Taif 21944, Saudi Arabia.
J AOAC Int. 2021 Sep 27;104(5):1415-1419. doi: 10.1093/jaoacint/qsab054.
Wrightia tinctoria R.Br. (Apocyanaceae) is known as a biologically effective plant for the treatment of jaundice in the Indian traditional system of medicine. It is a wild medicinal tree possessing anti-inflammatory, antidiabetic, antinociceptive, hepatoprotective, antibacterial, antifungal, antiviral, antipsoriatic, anticancerous, anthelmintic, aphrodisiac, analgesic, and antipyretic activities. Its constituents are of utmost interest to pharmaceutical industries owing to their many actions and biological activities.
Methanolic extract of W. tinctoria (MEWT) was investigated by gas chromatography-mass spectroscopy and provided affirmative results assisting in the identification and characterization of therapeutic claims regarding this species in the traditional system. The antioxidant activity of MEWT was determined by the most suitable DPPH method.
The basic compounds found in MEWT were β-caryophyllene (0.22%), mome inositol (12.02%), neophytadiene (1.61%), eicosanoic acid methyl ester (0.32%), 8,11,14-eicosatrienoic acid methyl ester (0.60%), phytol (0.94%), phytol palmitate (1.37%), squalene (1.57%), flavone 4-OH, 5-OH, 7-di-O-glucoside (29.34%), γ-tocopherol (0.49%), stigmast-5-en-3-ol (3.14%), methyl commate B (1.76%), methyl commate A (5.20%), and 24-norursa-3,12-diene (20.36%). The obtained results in the analysis of antioxidant activity of MEWT exhibited considerable free radical scavenging capacity against DPPH-generated free radicals.
This study expands the knowledge of MEWT chemical composition and provides evidence to substantiate ethno-medicinal use of the plant by exploring antioxidant activity. The substantial antioxidant activity of MEWT could be due to presence of terpenes, flavonoids, vitamin E, and other reported compounds.
This study includes identification of phytochemicals and antioxidant potential of methanolic extract of Wrightia tinctoria, assisting in therapeutic claims regarding this species in the traditional system.
Wrightia tinctoria R.Br.（夹竹桃科）在印度传统医学中被称为治疗黄疸的生物有效植物。它是一种野生药用树木，具有抗炎、抗糖尿病、抗伤害、保肝、抗菌、抗真菌、抗病毒、抗银屑病、抗癌、驱虫、壮阳、镇痛和解热作用。由于其多种作用和生物活性，其成分引起了制药行业的极大兴趣。
通过气相色谱-质谱联用技术对 Wrightia tinctoria 的甲醇提取物（MEWT）进行了研究，为该物种在传统系统中的治疗声称提供了肯定的鉴定和特征描述结果。通过最适合的 DPPH 法测定了 MEWT 的抗氧化活性。
MEWT 中发现的基本化合物为β-石竹烯（0.22%）、肌醇（12.02%）、新植二烯（1.61%）、二十烷酸甲酯（0.32%）、8,11,14-二十碳三烯酸甲酯（0.60%）、叶绿醇（0.94%）、叶绿醇棕榈酸酯（1.37%）、角鲨烯（1.57%）、黄酮 4-OH、5-OH、7-二-O-葡萄糖苷（29.34%）、γ-生育酚（0.49%）、豆甾-5-烯-3-醇（3.14%）、甲基邻苯二甲酸甲酯 B（1.76%）、甲基邻苯二甲酸甲酯 A（5.20%）和 24-降 Ursa-3,12-二烯（20.36%）。MEWT 抗氧化活性分析的结果表明，它对 DPPH 产生的自由基具有相当强的清除能力。
本研究扩展了 MEWT 化学成分的知识，并通过探索抗氧化活性为植物的民族医学用途提供了证据。MEWT 具有显著的抗氧化活性可能是由于萜类化合物、类黄酮、维生素 E 和其他报道的化合物的存在。
本研究包括鉴定 Wrightia tinctoria 甲醇提取物中的植物化学成分和抗氧化潜力，为该物种在传统系统中的治疗声称提供帮助。
