Mamedov L A, Gudz' T I, Nikolaev A V, Zakharov V V, Goncharenko E B
Biull Eksp Biol Med. 1988 May;105(5):628-30.
Newer method of thermofluorescence developed and experimentally used for identification of lipid peroxidation (LPO) products in the blood serum implied the application of 0.02 ml of studied serum onto a paper filter small disk (8mm in diameter) which was placed then on the cuvette of -38 device supplied with a heating element and thermopara. When thermopara was switched on and the blind opened, the cuvette was heated up to 120 degrees C with the heating rate of 60 degrees C per minute. A two-minute recording of fluorescence was simultaneously performed by "K4-003" self-recorder. By measuring the height of the obtained curve peak the intensity of LPO product fluorescence was determined in conditional units (one conditional unit was equal to one cm of curve peak height). A newer method identifying LPO products in the blood serum was more sensitive and time-sparing.
一种新开发的热荧光方法已被实验性地用于识别血清中的脂质过氧化（LPO）产物，该方法是将0.02毫升待测血清滴加到一张纸质滤盘小圆盘（直径8毫米）上，然后将其放置在配备加热元件和温差电偶的-38℃装置的比色皿中。当温差电偶开启且挡板打开时，比色皿以每分钟60℃的加热速率加热至120℃。同时通过“K4 - 003”自记录仪进行两分钟的荧光记录。通过测量所得曲线峰值的高度，以条件单位确定LPO产物荧光的强度（一个条件单位等于曲线峰值高度1厘米）。一种识别血清中LPO产物的新方法更灵敏且节省时间。
