Li Yanyun, Liao Shuting, Mei Pengying, Pan Yueyun, Zhang Yu, Zheng Xiangzi, Xie Yakun, Miao Ying
Fujian Provincial Key Laboratory of Plant Functional Biology, Fujian Agriculture and Forestry University, Fuzhou, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Plant Sci. 2021 Mar 22;12:643011. doi: 10.3389/fpls.2021.643011. eCollection 2021.
Cross talking between natural senescence and cell death in response to pathogen attack is an interesting topic; however, its action mechanism is kept open. In this study, 33 genes were obtained by screening with leaf aging procedure through RNA-seq dataset, and 11 of them were confirmed a significant altered expression level in the flag leaves during aging by using the reverse transcript quantitative PCR (RT-qPCR). Among them, the , , , , and members exhibited short-term alteration in transcriptional levels in response to infection. The CRISPR/Cas9-edited mutants of five genes were developed and confirmed, and a significant sensitivity to infection was observed in lines; on the other hand, a significant resistance to infection was shown in the enhanced expression plants compared to mock plants; however, enhanced expression of other four genes have no significant affection. Interestingly, ROS accumulation was also increased in enhanced plants after flg22 treatment, compared with the controls, suggesting that is involved in PAMP-triggered immune response in rice. It indicated that was involved in both flag leaf senescence and in response to fungi attack.
自然衰老与病原体攻击响应中的细胞死亡之间的相互作用是一个有趣的话题；然而，其作用机制尚不清楚。在本研究中，通过RNA-seq数据集利用叶片衰老程序筛选获得了33个基因，其中11个基因通过逆转录定量PCR（RT-qPCR）证实其在衰老过程中旗叶中的表达水平发生了显著变化。其中， 、 、 、 和 成员在响应 感染时转录水平呈现短期变化。开发并证实了五个基因的CRISPR/Cas9编辑突变体，在 株系中观察到对 感染的显著敏感性；另一方面，与模拟植株相比，增强表达 的植株对 感染表现出显著抗性；然而，其他四个基因的增强表达没有显著影响。有趣的是，与对照相比，flg22处理后 增强植株中的活性氧积累也增加，表明 参与了水稻中PAMP触发的免疫反应。这表明 既参与旗叶衰老又参与对真菌攻击的响应。
