Department of Kinesiology, University of Guelph-Humber, Toronto, ON, M9W 5L7, Canada.
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, N1G2W1, Canada.
Prostaglandins Leukot Essent Fatty Acids. 2021 May;168:102268. doi: 10.1016/j.plefa.2021.102268. Epub 2021 Mar 31.
Measuring fatty acid (FA) levels in blood as a risk factor for chronic disease has been studied extensively. Previous research has used either plasma or serum samples to examine these associations. However, whether results from plasma and serum samples can be compared remains unclear, as differences in methodology related to the separation of plasma and serum from whole blood may impact FA levels. This study analyzed the individual FA content of matched plasma and serum samples in both absolute (μg/mL) and relative percent (%) composition. Analyses were performed using archived fasted morning samples from the Florey Adelaide Male Ageing Study (FAMAS). Matched plasma and serum samples were available from 98 male subjects aged 40-85. Total FA were analyzed by gas-liquid chromatography equipped with a flame ionization detector (GLC-FID). Analyses comprised of over 60 FA including major FA such as Palmitic Acid (PA), Palmitoleic acid (POA), Stearic Acid (SA), Oleic Acid (OA), Linoleic Acid (LNA), alpha-linolenic acid (ALA), Eicosapentaenoic acid (EPA), Arachidonic Acid (ARA), and Docosahexaenoic acid (DHA). Differences between groups was determined by t-test. Correlation and Bland-Altman analyses were also performed to examine the relationship between plasma and serum samples. There were no significant differences between major plasma and serum fatty acids expressed in μg/mL and relative % composition. Correlation analysis determined a strong and significantly positive association (r ≥ 0.65, p < 0.05) between major plasma and serum FA in absolute and relative terms. Bland-Altman analysis further supported the strong agreement between plasma and serum values in both absolute and relative terms. These findings demonstrate that studies reporting plasma or serum fatty acid analyzed by GLC-FID can be compared with one another.
血液中脂肪酸（FA）水平作为慢性疾病的风险因素已经得到了广泛研究。先前的研究使用血浆或血清样本来检验这些关联。然而，血浆和血清样本的结果是否可以进行比较尚不清楚，因为与全血中血浆和血清分离相关的方法学差异可能会影响 FA 水平。本研究分析了来自弗洛里阿德莱德男性衰老研究（FAMAS）的禁食清晨存档样本中匹配的血浆和血清样本中的个体 FA 含量，包括绝对（μg/mL）和相对百分比（%）组成。匹配的血浆和血清样本来自 98 名年龄在 40-85 岁的男性。总 FA 通过配备火焰电离检测器（GLC-FID）的气液色谱法进行分析。分析包括超过 60 种 FA，包括主要 FA，如棕榈酸（PA）、棕榈油酸（POA）、硬脂酸（SA）、油酸（OA）、亚油酸（LNA）、α-亚麻酸（ALA）、二十碳五烯酸（EPA）、花生四烯酸（ARA）和二十二碳六烯酸（DHA）。通过 t 检验确定组间差异。还进行了相关性和 Bland-Altman 分析，以检验血浆和血清样本之间的关系。以 μg/mL 和相对%组成表示的主要血浆和血清脂肪酸之间没有显著差异。相关性分析确定了主要血浆和血清 FA 在绝对和相对术语中具有强烈且显著的正相关（r≥0.65，p<0.05）。Bland-Altman 分析进一步支持了绝对和相对术语中血浆和血清值之间的强烈一致性。这些发现表明，报告通过 GLC-FID 分析的血浆或血清脂肪酸的研究可以相互比较。
