Pan Meng-Hao, Wu Yu-Ke, Liao Bi-Yun, Zhang Hui, Li Chan, Wang Jun-Li, Hu Lin-Lin, Ma Baohua
Key Laboratory of Animal Biotechnology, Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Life Sciences Institute, Zhejiang University, Hangzhou, China.
Front Cell Dev Biol. 2021 Mar 23;9:661155. doi: 10.3389/fcell.2021.661155. eCollection 2021.
Bisphenol A (BPA) is one of the ubiquitous environmental endocrine disruptors (EEDs). Previous studies have shown that the reproduction toxicity of BPA could cause severe effects on the mammal oocytes and disturb the quality of mature oocytes. However, the toxic effects of BPA on the organelles of mouse oocytes have not been reported. In this study, to investigate whether BPA can be toxic to the organelles, we used different concentrations of BPA (50, 100, and 200 μM) to culture mouse oocytes . The results showed that 100 μM BPA exposure could significantly decrease the developmental capacity of oocytes. Then, we used the immunofluorescence staining, confocal microscopy, and western blotting to investigate the toxic effects of BPA on the organelles. The results revealed that mitochondrial dysfunction is manifested by abnormal distribution and decreased mitochondrial membrane potential. Moreover, the endoplasmic reticulum (ER) is abnormally distributed which is accompanied by ER stress showing increased expression of GRP78. For the Golgi apparatus, BPA-exposed dose not disorder the Golgi apparatus distribution but caused abnormal structure of Golgi apparatus, which is manifested by the decrease of GM130 protein expression. Moreover, we also found that BPA-exposed led to the damage of lysosome, which were shown by the increase of LAMP2 protein expression. Collectively, our findings demonstrated that the exposure of BPA could damage the normal function of the organelles, which may explain the reduced maturation quality of oocytes.
双酚A（BPA）是一种普遍存在的环境内分泌干扰物（EEDs）。先前的研究表明，BPA的生殖毒性可对哺乳动物卵母细胞造成严重影响，并扰乱成熟卵母细胞的质量。然而，BPA对小鼠卵母细胞细胞器的毒性作用尚未见报道。在本研究中，为了探究BPA是否对细胞器有毒性，我们使用不同浓度的BPA（50、100和200μM）培养小鼠卵母细胞。结果表明，暴露于100μM BPA可显著降低卵母细胞的发育能力。然后，我们使用免疫荧光染色、共聚焦显微镜和蛋白质印迹法来研究BPA对细胞器的毒性作用。结果显示，线粒体功能障碍表现为分布异常和线粒体膜电位降低。此外，内质网（ER）分布异常，并伴有内质网应激，表现为GRP78表达增加。对于高尔基体，暴露于BPA不会扰乱高尔基体的分布，但会导致高尔基体结构异常，表现为GM130蛋白表达减少。此外，我们还发现暴露于BPA会导致溶酶体损伤，表现为LAMP2蛋白表达增加。总的来说，我们的研究结果表明，暴露于BPA会损害细胞器的正常功能，这可能解释了卵母细胞成熟质量下降的原因。
