Delves P J, Roitt I M
Department of Immunology, University College and Middlesex School of Medicine, London, UK.
Clin Exp Immunol. 1988 Mar;71(3):459-63.
Isoelectric focusing of serial bleeds from patients with Hashimoto's thyroiditis was carried out and thyroglobulin (Tg) autoantibodies visualized using 125I-labelled Tg followed by autoradiography. Although the spectrotype of these antibodies was polyclonal and varied from patient to patient, each individual's spectrotype remained constant during the disease. Similar results were obtained if immunoblots were stained with rabbit anti-idiotype (anti-Id) raised to these autoantibodies. Using radioimmunoassay (RIA), it is shown that the levels of Id remain constant over several years whether assayed on crude immunoglobulin (Ig) fractions or affinity-purified anti-Tg. Therefore, once established, the autoimmune disease appears to be stable in terms of autoantibody spectrotype and idiotype in the patients studied.
对桥本甲状腺炎患者的系列出血样本进行等电聚焦，并使用¹²⁵I标记的甲状腺球蛋白（Tg）可视化Tg自身抗体，随后进行放射自显影。尽管这些抗体的光谱类型是多克隆的，且因患者而异，但在疾病过程中，每个个体的光谱类型保持不变。如果用针对这些自身抗体产生的兔抗独特型（抗Id）对免疫印迹进行染色，也会得到类似结果。使用放射免疫测定法（RIA）表明，无论对粗免疫球蛋白（Ig）组分还是亲和纯化的抗Tg进行检测，Id水平在数年中都保持恒定。因此，在所研究的患者中，一旦自身免疫性疾病确立，就自身抗体光谱类型和独特型而言，该疾病似乎是稳定的。
