Semenova N V, Madaeva I M, Bairova T A, Kolesnikova L I
Scientific Centre for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Zh Nevrol Psikhiatr Im S S Korsakova. 2021;121(3):93-97. doi: 10.17116/jnevro202112103193.
A comparative analysis of the parameters of lipid peroxidation - antioxidant defense system in menopausal women with- and without insomnia, depending on the polymorphism.
The study included 181 menopausal Caucasian women (ethnic group - Russians), who according to the results of the clinical-anamnestic examinations were divided into insomnia group (=120, age 53.68±4.61 years, body mass index 26.47±3.73 kg/m) and control group (=61, age 52.64±4.91 years, body mass index 27.69±3.11 kg/m). The insomnia group was formed according to the clinical guidelines for the diagnosis and treatment of chronic insomnia in adults, and scores of the Insomnia Severity Index. The study of the GSTT1 gene polymorphism was carried out using polymerase chain reaction. The lipid peroxidation - antioxidant system parameters were determined by spectrofluorophotometric methods.
In the insomnia group, carriers of the normal genotype had higher diene conjugates with lower glutathione peroxidase activity as compared to controls. When comparing groups of women with the deletion in an increase in the content of substrates and lipid peroxidation products was observed at all stages of lipid peroxidation, oxidized glutathione, glutathione S-transferase activity with a decrease in the content of reduced glutathione and retinol in the insomnia group as compared to controls. In the insomnia group, carriers of the deletion genotype had a higher content of ketodienes/conjugated trienes and active products of thiobarbituric acid, a lower content of retinol and α-tocopherol as compared to the patients with normal genotype. Insomnia in the menopausal women is accompanied with the intensification of lipid peroxidation only in the carriers of the deletion genotype.
根据基因多态性，对有失眠和无失眠的绝经后女性脂质过氧化-抗氧化防御系统参数进行比较分析。
该研究纳入了181名绝经后白种女性（种族为俄罗斯人），根据临床-病史检查结果，她们被分为失眠组（n = 120，年龄53.68±4.61岁，体重指数26.47±3.73kg/m²）和对照组（n = 61，年龄52.64±4.91岁，体重指数27.69±3.11kg/m²）。失眠组是根据成人慢性失眠诊断和治疗的临床指南以及失眠严重程度指数评分形成的。采用聚合酶链反应对GSTT1基因多态性进行研究。脂质过氧化-抗氧化系统参数通过分光荧光光度法测定。
在失眠组中，与对照组相比，正常基因型携带者的二烯共轭物含量较高，谷胱甘肽过氧化物酶活性较低。当比较有基因缺失的女性组时，与对照组相比，失眠组在脂质过氧化的所有阶段都观察到底物和脂质过氧化产物含量增加、氧化型谷胱甘肽、谷胱甘肽S-转移酶活性增加，而还原型谷胱甘肽和视黄醇含量降低。在失眠组中，与正常基因型患者相比，缺失基因型携带者的酮二烯/共轭三烯和硫代巴比妥酸活性产物含量较高，视黄醇和α-生育酚含量较低。绝经后女性的失眠仅在缺失基因型携带者中伴有脂质过氧化加剧。
