Federal State Public Scientific Institution "Scientific Centre for Family Health and Human Reproduction Problems" , Irkutsk , Russian Federation.
Chronobiol Int. 2019 Oct;36(10):1399-1408. doi: 10.1080/07420528.2019.1647436. Epub 2019 Aug 1.
A comparative analysis of lipid peroxidation processes and antioxidant defense system in Caucasian menopausal women with/without insomnia depending on the genotype of Clock 3111T/C gene polymorphism was performed. Two hundred and fourteen Caucasian menopausal women divided into control (without insomnia) and main group (with insomnia) were examined. Lipid peroxidation (conjugated dienes, thiobarbituric acid reactants) and antioxidant defense system parameters (?-tocopherol, retinol, reduced and oxidized glutathione, glutathione S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase) were determined by spectrofluorophotometer and immunoenzymometric methods. Patients with insomnia carriers of the TT-genotype had a significantly higher thiobarbituric acid reactants level and glutathione peroxidase activity as compared to group with insomnia carriers of the minor 3111C-allele ( < .05). A comparative analysis of the parameters in the women of the main and control groups showed higher conjugated dienes, thiobarbituric acid reactants levels and lower retinol, reduced glutathione levels, glutathione reductase activity in women with insomnia carriers of the TT-genotype ( < .05). The carriers of the minor allele with insomnia had a higher conjugated dienes levels and lower glutathione peroxidase activity as compared to control ( < .05). Thus, lipid peroxidation and antioxidant system parameters in Caucasian menopausal women with insomnia depend on the Clock 3111T/C gene polymorphism.
对高加索绝经妇女的脂质过氧化过程和抗氧化防御系统进行了比较分析，这些妇女患有/不患有失眠，且依据 Clock 3111T/C 基因多态性的基因型。检查了 214 名高加索绝经妇女，分为对照组（无失眠）和主要组（有失眠）。通过分光荧光光度计和免疫酶标法测定脂质过氧化（共轭二烯、硫代巴比妥酸反应物）和抗氧化防御系统参数（α-生育酚、视黄醇、还原和氧化型谷胱甘肽、谷胱甘肽 S-转移酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、超氧化物歧化酶）。与携带次要 3111C-等位基因的失眠患者相比，失眠的 TT 基因型携带者的硫代巴比妥酸反应物水平和谷胱甘肽过氧化物酶活性显著升高（<0.05）。对主要组和对照组妇女的参数进行比较分析显示，失眠的 TT 基因型携带者的共轭二烯、硫代巴比妥酸反应物水平较高，视黄醇、还原型谷胱甘肽水平较低，谷胱甘肽还原酶活性较低（<0.05）。与对照组相比，携带次要等位基因的失眠携带者的共轭二烯水平较高，谷胱甘肽过氧化物酶活性较低（<0.05）。因此，高加索绝经失眠妇女的脂质过氧化和抗氧化系统参数取决于 Clock 3111T/C 基因多态性。
