一种用于检测食用油中苯并[]芘的基于金的条带传感器。
A gold-based strip sensor for the detection of benzo[]pyrene in edible oils.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Standards & Quality Center of National Food and Strategic Reserves Administration, Xicheng District, 100037 Beijing, China.
出版信息
Analyst. 2021 Jun 14;146(12):3871-3879. doi: 10.1039/d1an00612f.
This report describes the development of a sensitive and broadly specific indirect competitive enzyme-linked immunosorbent assay (icELISA) and a gold nanoparticle-based immunochromatographic strip (GNP-ICS) assay for the detection of benzo[a]pyrene (B[a]P), using an anti-B[a]P monoclonal antibody (mAb). A broad-specific anti-B[a]P mAb (4E8) was raised from two types of haptens, with half maximal inhibitory concentrations and limits of detection (LOD) values of 2.51 and 0.54 ng mL-1, respectively. In addition, the cross-reactivity was up to 390% with structurally related compounds. The GNP-ICS assay based on a GNP-labeled mAb showed broad specificity in the detection of B[a]P and its analogues, with cut-off and visual LOD values of 100 and 10 ng mL-1, respectively. Furthermore, the recoveries from the developed icELISA and GNP-ICS assay in edible oil samples spiked with B[a]P were validated by high-performance liquid chromatography-fluorescence detection. The results revealed that the icELISA could reliably detect B[a]P in edible oils.
本报告描述了一种敏感且广谱特异性的间接竞争酶联免疫吸附测定（icELISA）和基于金纳米粒子的免疫层析条（GNP-ICS）检测方法的开发，用于检测苯并[a]芘（B[a]P），使用了一种抗 B[a]P 单克隆抗体（mAb）。通过两种半抗原，制备出了广谱特异性的抗 B[a]P mAb（4E8），其半数最大抑制浓度和检测限（LOD）值分别为 2.51 和 0.54 ng mL-1。此外，与结构相关的化合物的交叉反应性高达 390%。基于 GNP 标记 mAb 的 GNP-ICS 检测方法在检测 B[a]P 及其类似物时表现出广谱特异性，其截止值和可视 LOD 值分别为 100 和 10 ng mL-1。此外，通过高效液相色谱-荧光检测，对添加 B[a]P 的食用油样品中开发的 icELISA 和 GNP-ICS 检测方法的回收率进行了验证。结果表明，icELISA 可可靠地检测食用油中的 B[a]P。
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