Population Health Sciences, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.
Department of Neurology, Faculty of Medicine, University of Bonn, Bonn, Germany.
Nat Commun. 2021 Apr 9;12(1):2117. doi: 10.1038/s41467-021-22351-5.
To estimate the seroprevalence and temporal course of SARS-CoV-2 neutralizing antibodies, we embedded a multi-tiered seroprevalence survey within an ongoing community-based cohort study in Bonn, Germany. We first assessed anti-SARS-CoV-2 immunoglobulin G levels with an immunoassay, followed by confirmatory testing of borderline and positive test results with a recombinant spike-based immunofluorescence assay and a plaque reduction neutralization test (PRNT). Those with a borderline or positive immunoassay result were retested after 4 to 5 months. At baseline, 4771 persons participated (88% response rate). Between April 24 and June 30, 2020, seroprevalence was 0.97% (95% CI: 0.72-1.30) by immunoassay and 0.36% (95% CI: 0.21-0.61) when considering only those with two additional positive confirmatory tests. Importantly, about 20% of PRNT+ individuals lost their neutralizing antibodies within five months. Here, we show that neutralizing antibodies are detectable in only one third of those with a positive immunoassay result, and wane relatively quickly.
为了估计 SARS-CoV-2 中和抗体的血清阳性率和时间进程，我们在德国波恩的一项正在进行的基于社区的队列研究中嵌入了一个多层次的血清阳性率调查。我们首先使用免疫测定法评估抗 SARS-CoV-2 免疫球蛋白 G 水平，然后使用基于重组刺突的免疫荧光测定法和蚀斑减少中和试验（PRNT）对边界和阳性检测结果进行确认性检测。那些免疫测定法结果为边界或阳性的人在 4 到 5 个月后进行了复测。在基线时，有 4771 人参加（88%的应答率）。在 2020 年 4 月 24 日至 6 月 30 日期间，免疫测定法的血清阳性率为 0.97%（95%CI：0.72-1.30），当仅考虑另外两项阳性确认性检测时，血清阳性率为 0.36%（95%CI：0.21-0.61）。重要的是，大约 20%的 PRNT+个体在五个月内失去了中和抗体。在这里，我们表明，在免疫测定法阳性结果的人中，只有三分之一可检测到中和抗体，并且很快衰减。
