Irmak Gülseren, Demirtaş Tuǧrul Tolga, Gümüşderelioǧlu Menemşe
ACS Biomater Sci Eng. 2019 Feb 11;5(2):831-845. doi: 10.1021/acsbiomaterials.8b00778. Epub 2018 Dec 26.
Methacrylated gelatin (Gel-MA) is a commonly used biomaterial in bioprinting applications. The Gel-MA synthesis procedure is inadequate and needs to be improved, particularly from the point of optimization and efficacy. We report a significantly faster (by 5 min) and effective method to controllably synthesize Gel-MA using microwave energy (Mw at 1000 W power) with ≥90% degree of methacrylation (DM) even with the use of a very low concentration of methacrylic anhydride (MA). Rheological and mechanical analyses indicated that Gel-MA synthesized by Mw-assisted methacrylation enabled the formation of hydrogels that are more elastic and stronger and have a lower degradation rate (∼27% at 35 days) than Gel-MA synthesized by the conventional method. The viscosity values of the Gel-MA bioink were in the range applicable for use in 3D bioprinters. Additionally, Mw-assisted methacrylated Gel-MA hydrogels that have mechanically superior properties significantly enhanced the viability, attachment, proliferation, alkaline phosphatase (ALP) activity, mineral deposition, and mRNA expression levels of osteogenic genes of MC3T3-E1 preosteoblastic cells.
甲基丙烯酸化明胶(Gel-MA)是生物打印应用中常用的生物材料。Gel-MA的合成过程存在不足,需要改进,特别是在优化和效果方面。我们报告了一种显著更快(快5分钟)且有效的方法,使用微波能量(功率为1000W的微波)可控地合成Gel-MA,即使使用非常低浓度的甲基丙烯酸酐(MA),甲基丙烯酸化程度(DM)也≥90%。流变学和力学分析表明,通过微波辅助甲基丙烯酸化合成的Gel-MA能够形成比传统方法合成的Gel-MA更具弹性、更强且降解率更低(35天时约为27%)的水凝胶。Gel-MA生物墨水的粘度值在适用于3D生物打印机的范围内。此外,具有机械性能优越的微波辅助甲基丙烯酸化Gel-MA水凝胶显著提高了MC3T3-E1前成骨细胞的活力、附着、增殖、碱性磷酸酶(ALP)活性、矿物质沉积以及成骨基因的mRNA表达水平。