University of Granada, Faculty of Dentistry, Colegio Máximo de Cartuja s/n, 18071, Granada, Spain.
University of Granada, Faculty of Dentistry, Colegio Máximo de Cartuja s/n, 18071, Granada, Spain.
Dent Mater. 2021 Jul;37(7):1107-1120. doi: 10.1016/j.dental.2021.03.007. Epub 2021 Apr 10.
To investigate the effectiveness of novel polymeric nanoparticles (NPs) doped with melatonin (ML) in reducing dentin permeability and facilitating dentin remineralization after endodontic treatment.
The effect of undoped NPs and ML-doped NPs (ML-NPs) was tested in radicular dentin, at 24 h and 6 m. A control group without NPs was included. ML liberation was measured. Radicular dentin was assessed for fluid filtration. Dentin remineralization was analyzed by scanning electron microscopy, AFM, Young's modulus (Ei), Nano DMA-tan delta, and Raman analysis.
ML release ranged from 1.85 mg/mL at 24 h to 0.033 mg/mL at 28 d. Both undoped NPs and ML-NPs treated dentin exhibited the lowest microleakage, but samples treated with ML-NPs exhibited hermetically sealed dentinal tubules and extended mineral deposits onto dentin. ML-NPs promoted higher and durable Ei, and functional remineralization at root dentin, generating differences between the values of tan delta among groups and creating zones of stress concentration. Undoped-NPs produced closure of some tubules and porosities at the expense of a relative mineral amorphization. Chemical remineralization based on mineral and organic assessments was higher in samples treated with ML-NPs. When using undoped NPs, precipitation of minerals occurred; however, radicular dentin was not mechanically reinforced but weakened over time.
Application of ML-NPs in endodontically treated teeth, previous to the canal filling step, is encouraged due to occlusion of dentinal tubules and the reinforcement of the radicular dentin structure.
研究新型聚合物纳米粒子（NPs）掺杂褪黑素（ML）在减少根管治疗后牙本质渗透性和促进牙本质再矿化的效果。
在根管牙本质中，在 24 小时和 6 个月时测试未掺杂 NPs 和 ML 掺杂 NPs（ML-NPs）的效果。包括无 NPs 的对照组。测量 ML 释放。评估根管牙本质的流体过滤。通过扫描电子显微镜、原子力显微镜、杨氏模量（Ei）、Nano DMA-tan delta 和拉曼分析分析牙本质再矿化。
ML 释放量从 24 小时的 1.85 mg/mL 到 28 天的 0.033 mg/mL。未掺杂 NPs 和 ML-NPs 处理的牙本质均表现出最低的微渗漏，但用 ML-NPs 处理的样本表现出密封的牙本质小管和延伸到牙本质上的矿物质沉积。ML-NPs 促进了根牙本质更高和更持久的 Ei 和功能再矿化，导致各组之间 tan delta 值的差异，并形成了应力集中区。未掺杂-NPs 以相对的矿物质非晶化为代价，封闭了一些小管和孔隙。基于矿物质和有机评估的化学再矿化在 ML-NPs 处理的样本中更高。使用未掺杂 NPs 时，矿物质沉淀发生；然而，随着时间的推移，根牙本质不仅没有得到机械增强，反而被削弱了。
由于牙本质小管的闭塞和根牙本质结构的增强，在根管填充步骤之前，将 ML-NPs 应用于根管治疗后的牙齿是被鼓励的。
