Zhang Xin, Zhang Yuxuan, Li Ying, Wang Xiaoming, Zhang Xueqin
College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China.
FuYang Sineva Materials Technology Co., Ltd., Beijing 100176, China.
Materials (Basel). 2023 Sep 29;16(19):6497. doi: 10.3390/ma16196497.
The ability of dental materials to induce the mineralization of enamel like hydroxyapatite (HA) is of great importance. In this article, a novel kind of dental restorative material characterized by a mineralization ability was fabricated by photopolymerization. Calcium methacrylate (CMA) was introduced into the classical bisphenol A-glycidyl methacrylate (Bis-GMA) and triethylene glycol dimethacrylate (TEGDMA) dental resin formulation. This functional dental resin (BTCM) was calcium-rich and can be prepared simply by one-step photopolymerization. The influence of CMA on the photopolymerization kinetics, the dental resin's mechanical properties, and its capacity to induce dynamic in situ HA mineralization were examined. Real-time FTIR, compression modulus, scanning electron microscopy, X-ray spectroscopy, MTT assay, and cell attachment test were carried out. The obtained data were analyzed for statistical significance using analysis of variance (ANOVA). Double bond conversion could be completed in less than 300 s, while the compression modulus of BTCM decreased with the increase in CMA content (30 wt%, 40 wt%, and 50 wt%). After being soaked in Ca(NO) and NaHPO solutions alternatively, dense HA crystals were found on the surface of the dental resin which contained CMA. The amount of HA increased with the increase in CMA content. The MTT results indicated that BTCM possesses good biocompatibility, while the cell adhesion and proliferation investigation demonstrated that L929 cells can adhere and proliferate well on the surface of BTM. Thus, our approach provides a straightforward, cost-effective, and environmentally friendly solution that has the potential for immediate clinical use.
牙科材料诱导形成类似羟基磷灰石（HA）的牙釉质矿化的能力非常重要。在本文中，通过光聚合制备了一种具有矿化能力的新型牙科修复材料。将甲基丙烯酸钙（CMA）引入经典的双酚A-甲基丙烯酸缩水甘油酯（Bis-GMA）和二缩三乙二醇二甲基丙烯酸酯（TEGDMA）牙科树脂配方中。这种功能性牙科树脂（BTCM）富含钙，并且可以通过一步光聚合简单制备。研究了CMA对光聚合动力学、牙科树脂力学性能及其诱导动态原位HA矿化能力的影响。进行了实时傅里叶变换红外光谱、压缩模量、扫描电子显微镜、X射线光谱、MTT试验和细胞附着试验。使用方差分析（ANOVA）对获得的数据进行统计学显著性分析。双键转化可在不到300秒内完成，而BTCM的压缩模量随CMA含量（30 wt%、40 wt%和50 wt%）的增加而降低。在交替浸泡于Ca(NO)和NaHPO溶液后，在含有CMA的牙科树脂表面发现了致密的HA晶体。HA的量随CMA含量的增加而增加。MTT结果表明BTCM具有良好的生物相容性，而细胞粘附和增殖研究表明L929细胞可以在BTM表面良好地粘附和增殖。因此，我们的方法提供了一种直接、经济高效且环境友好的解决方案，具有立即临床应用的潜力。
