Wahl R L, Liebert M, Wilson B S, Petry N A
University of Michigan Medical Center, Department of Internal Medicine, Ann Arbor 48109-0028.
Int J Rad Appl Instrum B. 1988;15(3):243-50. doi: 10.1016/0883-2897(88)90102-x.
The kinetics of lymph node and systemic uptake of members of three different classes of lymphoscintigraphic agents were studied in normal laboratory rats. 99mTc antimony trisulfide colloid (TcSbC), 99mTc human serum albumin (TcHSA), 125I 5G6.4 (a murine IgG2ak monoclonal antibody), 125I 763.24T (a murine IgG1), and 125I FT166 ( a murine IgM monoclonal) all current or potential lymphoscintigraphic agents, were injected subcutaneously into the hind foot pads of healthy rats. Ipsilateral and contralateral popliteal lymph nodes were sampled up to 4 h post-injection. Subcutaneous injection resulted in far higher nodal uptake for all agents than i.v. delivery with ipsilateral popliteal node/blood ratios 1 h postsubcutaneous injection of: for TcSbC (1900) greater than 125I IgM (497) greater than TcHSA (72) greater than 125I Intact IgG2a or 125I IgG1 at approximately 10. Thus, while all agents achieve popliteal node/blood ratios far greater than unity, TcSbc has the greatest absolute and relative nodal accumulation, greater than the 125I IgM monoclonal antibody and TcHSA. Uptake of the intact 125I IgG antibodies is lowest. These data suggest that TcSbC in particular, as well as TcHSA and IgM may be most useful as non-specific nodel imaging agents, while the lower background activity of the IgGs may make targeting specific antigen in nodes more feasible.
在正常实验大鼠中研究了三类不同淋巴闪烁造影剂的淋巴结摄取动力学和全身摄取情况。99mTc 三硫化锑胶体（TcSbC）、99mTc 人血清白蛋白（TcHSA）、125I 5G6.4（一种鼠 IgG2ak 单克隆抗体）、125I 763.24T（一种鼠 IgG1）以及 125I FT166（一种鼠 IgM 单克隆抗体），这些都是目前或潜在的淋巴闪烁造影剂，被皮下注射到健康大鼠的后足垫中。在注射后长达 4 小时对同侧和对侧腘窝淋巴结进行取样。皮下注射导致所有试剂的淋巴结摄取远高于静脉注射，皮下注射后 1 小时同侧腘窝淋巴结/血液比值为：TcSbC（1900）大于 125I IgM（497）大于 TcHSA（72）大于 125I 完整 IgG2a 或 125I IgG1，约为 10。因此，虽然所有试剂的腘窝淋巴结/血液比值都远大于 1，但 TcSbc 的绝对和相对淋巴结积累最大，大于 125I IgM 单克隆抗体和 TcHSA。完整的 125I IgG 抗体的摄取最低。这些数据表明，特别是 TcSbC，以及 TcHSA 和 IgM 可能作为非特异性淋巴结成像剂最有用，而 IgG 的较低本底活性可能使在淋巴结中靶向特定抗原更可行。
