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sp. nov.,从一名免疫功能低下患者的血液中分离得到。

sp. nov., isolated from blood of an immunocompromised patient.

机构信息

Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

Department of Life Science, Chung Ang University, Seoul, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004759.

Abstract

A novel Gram-stain-negative, facultative aerobic and rod-shaped bacterium, designated as MKL-01 and isolated from the blood of immunocompromised patient, was genotypically and phenotypically characterized. The colonies were found to be creamy yellow and convex. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that strain MKL-01 was most closely related to LMG 5886, present within a large cluster in the genus . The genome sequence of strain MKL-01 showed the highest average nucleotide identity value of 92.1 % and digital DNA-DNA hybridization value of 44.8 % with the closely related species LMG 5886. The genome size of the isolate was 5 750 268 bp, with a G+C content of 67.87 mol%. The strain could grow at 10-45 °C (optimum, 37-40 °C), in the presence of 0-10 % (w/v) NaCl (optimum, 0.5%) and at pH 6.0-10.0 (optimum, pH 7.0). Strain MKL-01 was positive for catalase and negative for oxidase. The major fatty acids were C, summed feature 3 (C 7/C 6 and/or C 6/C 7) and summed feature 8 (C 7 and/or C 6). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and one unidentified polar lipid. Moreover, strain MKL-01 contained ubiquinone Q-8 as the sole respiratory quinone. Based on its molecular, phenotypic and chemotaxonomic properties, strain MKL-01 represents a novel species of the genus ; the name sp. nov. is proposed for this strain. The type strain is MKL-01.

摘要

一株新型革兰氏阴性、兼性需氧、杆状细菌,被命名为 MKL-01,从免疫功能低下患者的血液中分离得到,对其进行了基因型和表型特征描述。该菌的菌落呈奶油黄色、凸起。基于 16S rRNA 基因和全基因组序列的系统发育分析表明,菌株 MKL-01与 LMG 5886 最为密切相关,属于一个大的属内聚类。MKL-01 菌株的基因组序列与密切相关的种 LMG 5886 的平均核苷酸同一性值最高为 92.1%,数字 DNA-DNA 杂交值为 44.8%。该分离株的基因组大小为 5750268bp,G+C 含量为 67.87mol%。该菌株可在 10-45°C(最佳温度为 37-40°C)、0-10%(w/v)NaCl(最佳浓度为 0.5%)和 pH6.0-10.0(最佳 pH 值为 7.0)下生长。MKL-01 菌株阳性触酶,阴性氧化酶。主要脂肪酸为 C 、特征 3(C 7/C 6 和/或 C 6/C 7)和特征 8(C 7 和/或 C 6)。极性脂谱由磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷脂和一种未鉴定的极性脂组成。此外,菌株 MKL-01 含有泛醌 Q-8 作为唯一的呼吸醌。基于其分子、表型和化学分类学特性,菌株 MKL-01 代表了属的一个新种,建议将该菌株命名为 sp. nov. 。模式菌株为 MKL-01。

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