Brasseur P, Lemeteil D, Ballet J J
Department of Parasitology, Hôtel Dieu, Centre Hospitalier Universitaire, Rouen, France.
J Clin Microbiol. 1988 May;26(5):1037-9. doi: 10.1128/jcm.26.5.1037-1039.1988.
Effective treatment for Cryptosporidium infection in immunocompromised patients has yet to be found. We report a rodent model of persistent Cryptosporidium infection. Sprague-Dawley rats were injected subcutaneously twice a week for 8 weeks with 25 mg of hydrocortisone acetate. Fed a regular low-protein diet for 9 weeks, they were challenged once with 10(5) calf Cryptosporidium oocysts 5 weeks after the start of the hydrocortisone acetate regimen. Oocyst shedding was evaluated in feces daily by using a carbolfuchsin-staining method. Rats shed oocysts from days 2 to 9 after ingestion and developed a persistent infection for more than 38 days. Excretion was lower after subsequent parasite challenges, suggesting that a degree of protection developed progressively. The results suggest that this experimental model provides a procedure for screening candidate therapeutic agents.
尚未找到针对免疫功能低下患者隐孢子虫感染的有效治疗方法。我们报告了一种持续性隐孢子虫感染的啮齿动物模型。将Sprague-Dawley大鼠每周皮下注射两次25毫克醋酸氢化可的松，持续8周。在醋酸氢化可的松给药方案开始5周后，给它们喂食常规低蛋白饮食9周，然后用10(5)个小牛隐孢子虫卵囊攻击一次。每天通过使用石炭酸品红染色法评估粪便中的卵囊排出情况。大鼠在摄入卵囊后第2天至第9天排出卵囊，并发展为持续感染超过38天。后续寄生虫攻击后排泄物减少，表明逐渐产生了一定程度的保护作用。结果表明，该实验模型为筛选候选治疗药物提供了一个程序。
