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用于检测运动性沙门氏菌的新型半固体琼脂。

New semisolid agar for the detection of motile salmonellae.

作者信息

Hine E A, Stefen E K, Wagner J E

机构信息

Research Animal Diagnostic and Investigative Laboratory, College of Veterinary Medicine, University of Missouri, Columbia 65211.

出版信息

J Clin Microbiol. 1988 May;26(5):875-8. doi: 10.1128/jcm.26.5.875-878.1988.

Abstract

A semisolid selective motility agar based on selenite broth and designated semisolid selenite fecal (SSF) agar was developed for recovery of motile salmonellae from large numbers of rodent fecal specimens. The medium is easily prepared and inoculated; results are readily interpreted. When combined with selenite or gram-negative broth enrichment, SSF agar yielded significantly better Salmonella isolation from experimentally infected rodents than a standard battery of media. Only 14 non-Salmonella isolates from 1,002 Salmonella-free rodent fecal specimens migrated on SSF agar.

摘要

一种基于亚硒酸盐肉汤、名为半固体亚硒酸盐粪便(SSF)琼脂的半固体选择性动力琼脂被开发出来,用于从大量啮齿动物粪便标本中分离运动性沙门氏菌。该培养基易于制备和接种;结果易于解读。当与亚硒酸盐或革兰氏阴性肉汤增菌培养相结合时,SSF琼脂从实验感染的啮齿动物中分离沙门氏菌的效果明显优于一套标准培养基。在1002份无沙门氏菌的啮齿动物粪便标本中,只有14株非沙门氏菌分离株在SSF琼脂上有迁徙现象。

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