使用Sensititre断点自动读数系统进行快速及过夜微量稀释抗生素敏感性试验。

Rapid and overnight microdilution antibiotic susceptibility testing with the Sensititre Breakpoint Autoreader system.

作者信息

Nolte F S, Krisher K K, Beltran L A, Christianson N P, Sheridan G E

机构信息

Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642.

出版信息

J Clin Microbiol. 1988 Jun;26(6):1079-84. doi: 10.1128/jcm.26.6.1079-1084.1988.

DOI:10.1128/jcm.26.6.1079-1084.1988

PMID:3384920

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266535/

Abstract

The Sensititre Breakpoint Autoreader system (SBAS) is a broth microdilution method with one to three concentrations of each antibiotic and innovative fluorescence technology to define inhibitory endpoints. We tested 248 gram-negative bacilli and 80 gram-positive cocci using both the rapid (5 h) and overnight (18 h) SBAS procedures. Inhibitory endpoints were also determined by visual inspection of the microdilution trays after 18 h of incubation. SBAS results were compared with those obtained by a standardized disk diffusion (SDD) procedure. Agreement between the rapid SBAS and SDD results for all antibiotic-organism combinations was found in 3,730 of 4,571 (81.6%) tests, with 3.9% very major, 6.5% major, and 7.9% minor discrepancies noted. Data analysis by organism group revealed 86.8, 57.3, 71.4, and 62.3% agreement for members of the family Enterobacteriaceae, Pseudomonas spp., staphylococci, and enterococci, respectively. The results of the overnight SBAS and SDD agreed in 4,154 of 4,654 (89.2%) tests, with 2.3% very major, 1.3% major, and 7.1% minor discrepancies recorded. Concordance was noted in 90.4, 78.1, 90.6, and 83.3% of the comparisons for the members of the Enterobacteriaceae, Pseudomonas spp., staphylococci, and enterococci, respectively. The inhibitory endpoints determined with the Autoreader were as reliable as those determined by visual inspection after 18 h of incubation.

摘要

Sensititre断点自动判读系统（SBAS）是一种肉汤微量稀释法，每种抗生素有一至三种浓度，并采用创新的荧光技术来确定抑制终点。我们使用快速（5小时）和过夜（18小时）SBAS程序对248株革兰氏阴性杆菌和80株革兰氏阳性球菌进行了测试。在孵育18小时后，还通过目视检查微量稀释板来确定抑制终点。将SBAS结果与通过标准化纸片扩散法（SDD）获得的结果进行比较。在4571次测试中的3730次（81.6%）测试中，发现快速SBAS和SDD结果对于所有抗生素-菌株组合均一致，其中发现3.9%为极重大差异、6.5%为重大差异、7.9%为微小差异。按菌株组进行数据分析显示，肠杆菌科、假单胞菌属、葡萄球菌和肠球菌成员的一致性分别为86.8%、57.3%、71.4%和62.3%。过夜SBAS和SDD的结果在4654次测试中的4154次（89.2%）测试中一致，记录的极重大差异为2.3%、重大差异为1.3%、微小差异为7.1%。在肠杆菌科、假单胞菌属、葡萄球菌和肠球菌成员的比较中，一致性分别为90.4%、78.1%、90.6%和83.3%。自动判读仪确定的抑制终点与孵育18小时后目视检查确定的抑制终点一样可靠。