Shanson D C, Dryden M S
St Stephen's Hospital, London.
J Clin Pathol. 1988 Jun;41(6):687-90. doi: 10.1136/jcp.41.6.687.
A variety of blood culture media were compared to determine the optimal method for the isolation of Mycobacterium avium-intracellulare (MAI) from the blood of patients with AIDS. Simulated laboratory blood cultures and clinical blood cultures were tested. Glucose broth, Bactec aerobic medium, Kirschner's medium, and Bactec Middlebrook medium supported the growth of MAI. The Isolator system, a lysis centrifugation method, facilitated the most rapid isolation of MAI (p = 0.001). The Bactec Middlebrook medium gave the most rapid detection rate (p = 0.001) as acid fast bacilli could be stained by the Ziehl-Neelson method before colonies were isolated in the Isolator system. The growth index readings did not reliably predict mycobacterial growth in the two radiometric media tested. Although several methods may be used to isolate MAI from blood, the most rapid and sensitive method is the Isolator system used in combination with the Bactec Middlebrook medium.
比较了多种血培养培养基，以确定从艾滋病患者血液中分离鸟分枝杆菌-胞内分枝杆菌（MAI）的最佳方法。对模拟实验室血培养和临床血培养进行了检测。葡萄糖肉汤、Bactec需氧培养基、基尔施纳培养基和Bactec Middlebrook培养基支持MAI生长。Isolator系统（一种裂解离心法）有助于最快速地分离MAI（p = 0.001）。Bactec Middlebrook培养基检测速度最快（p = 0.001），因为在Isolator系统中分离出菌落之前，抗酸杆菌可用齐尔-尼尔森法染色。在所测试的两种放射性测量培养基中，生长指数读数不能可靠地预测分枝杆菌的生长。虽然可以使用几种方法从血液中分离MAI，但最快速、灵敏的方法是将Isolator系统与Bactec Middlebrook培养基联合使用。
