鉴定和特征分析 × 中的基因家族及其在不利环境胁迫下的潜在作用。

Identification and Characterization of the Gene Family in × and Their Potential Role under Adverse Environmental Stresses.

机构信息

College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.

National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, Chengdu 611130, China.

出版信息

Int J Mol Sci. 2023 Aug 30;24(17):13465. doi: 10.3390/ijms241713465.

DOI:10.3390/ijms241713465

PMID:37686287

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10488121/

Abstract

Zinc finger protein (ZFP) transcription factors play a pivotal role in regulating plant growth, development, and response to biotic and abiotic stresses. Although extensively characterized in model organisms, these genes have yet to be reported in bamboo plants, and their expression information is lacking. Therefore, we identified 21 B-box () genes from a transcriptome analysis of . Consequently, multiple sequence alignments and an analysis of conserved motifs showed that they all had highly similar structures. The genes were divided into four subgroups according to their phylogenetic relationships and conserved domains. A GO analysis predicted multiple functions of the genes in photomorphogenesis, metabolic processes, and biological regulation. We assessed the expression profiles of 21 genes via qRT-PCR under different adversity conditions. Among them, eight genes were significantly up-regulated under water deficit stress (, , , , , , , and ), nine under salt stress (, , , , , , , , and ), twelve under cold stress (, , , , , , , , , , , and ), and twelve under pathogen infestation stress (, , , , , , , , , , , and ). Three genes (, , and ) were significantly up-regulated under both biotic and abiotic stresses. These results suggest that the gene family is integral to plant growth, development, and response to multivariate stresses. In conclusion, we have comprehensively analyzed the genes under various adversity stress conditions, thus providing valuable information for further functional studies of this gene family.

摘要

锌指蛋白（ZFP）转录因子在调节植物生长、发育和对生物及非生物胁迫的响应方面发挥着关键作用。尽管这些基因在模式生物中得到了广泛的研究，但在竹子植物中尚未被报道，其表达信息也缺乏。因此，我们从转录组分析中鉴定出 21 个 B-box()基因。

随后，通过多序列比对和保守基序分析表明，它们都具有高度相似的结构。根据系统发育关系和保守结构域，将 21 个基因分为四个亚组。GO 分析预测 21 个基因在光形态建成、代谢过程和生物调节等方面具有多种功能。

通过 qRT-PCR 检测了 21 个基因在不同逆境条件下的表达谱。其中，8 个基因在水分胁迫下显著上调（、、、、、和），9 个基因在盐胁迫下显著上调（、、、、、、和），12 个基因在冷胁迫下显著上调（、、、、、、、、和），12 个基因在病原体侵染胁迫下显著上调（、、、、、、、、和）。有 3 个基因（、和）在生物和非生物胁迫下均显著上调。

这些结果表明，基因家族在植物生长、发育和对多因素胁迫的响应中起着不可或缺的作用。总之，我们全面分析了基因在各种逆境胁迫条件下的表达情况，为进一步研究该基因家族的功能提供了有价值的信息。