人乳头瘤病毒、基因突变与乳腺癌的雌激素和孕激素受体：一项横断面研究。
Human papillomavirus, gene mutation and estrogen and progesterone receptors in breast cancer: a cross-sectional study.
机构信息
Faculty of Medicine, Batterjee Medical College of Science and Technology, Jeddah, KSA.
Histopathology and Cytology Department, Faculty of Graduate Study and Scientific Research, National Ribat University, Khartoum, Sudan.
出版信息
Pan Afr Med J. 2021 Jan 15;38:43. doi: 10.11604/pamj.2021.38.43.22013. eCollection 2021.
INTRODUCTION
recent studies show a good relationship between breast cancer (BC) and human papillomaviruses (HPV) wich is responsible for about 18% of BC cases. This study aimed to assess the relationship between different genotypes of HPV and the expression of P53 and retinoblastoma (RB) genes and estrogen and progesterone receptors in BC among Sudanese women.
METHODS
one hundred and fifty tissue blocks were obtained from females diagnosed with BC. Positive samples were used to determine genotypes with an applied biosystem (ABI 3730XL) genetic analyzer for sequencing and immunohistochemistry.
RESULTS
13/150 samples showed HPV DNA. High-risk HPV-16 was detected in 5 cases, high-risk-HPV-58 was found in four cases, and HPV-18 was detected in three cases. Low-risk-HPV-11 was detected in a single invasive lobular carcinoma (ILC) case. P53 and RB gene mutations were detected in 35 and 30 BC cases, respectively. P53 gene mutation was frequently identified in grade (III) BC while RB gene mutation was positive in grade (II). Grade (II) BC had a higher incidence of HPV-16 and 58. On the other hand, HPV-18 had a higher incidence in grade (III). Estrogen and progesterone receptors were expressed in 94 and 79 HPV cases among the study group, respectively.
CONCLUSION
this study elucidates the associations between HPV genotypes and BC. A statistically significant association was observed among p53 and RB gene mutations and different BC histological types. On the other hand, there was a statistically insignificant association between HPV genotyping and different BC gradings, BC histological types, P53 and RB genes mutations, and estrogen and progesterone receptor expression. Also, there was a statistically insignificant association among estrogen and progesterone receptors expression and BC grading. RB gene mutation was significantly associated with different BC grades. On the other hand, there was a statistically insignificant association between progesterone receptor expression and BC.
简介
最近的研究表明，乳腺癌（BC）与人类乳头瘤病毒（HPV）之间存在良好的关系，HPV 约占 BC 病例的 18%。本研究旨在评估苏丹女性中不同 HPV 基因型与 P53 和视网膜母细胞瘤（RB）基因表达以及雌激素和孕激素受体在 BC 中的关系。
方法
从诊断为 BC 的女性中获得了 150 个组织块。使用聚合酶链反应（PCR）从阳性样本中提取 HPV DNA，然后使用Applied Biosystems（ABI 3730XL）基因分析仪进行测序和免疫组织化学分析。
结果
在 150 个样本中，有 13 个样本检测到 HPV DNA。在 5 例中检测到高危型 HPV-16，在 4 例中检测到高危型 HPV-58，在 3 例中检测到 HPV-18。在一例浸润性小叶癌（ILC）中检测到低危型 HPV-11。在 35 例 BC 病例中检测到 P53 基因突变，在 30 例 BC 病例中检测到 RB 基因突变。P53 基因突变在 III 级 BC 中更为常见，而 RB 基因突变在 II 级 BC 中呈阳性。在 II 级 BC 中，HPV-16 和 58 的发生率较高，而 HPV-18 在 III 级 BC 中发生率较高。在研究组中，94 例和 79 例 HPV 病例分别表达了雌激素和孕激素受体。
结论
本研究阐明了 HPV 基因型与 BC 之间的关系。在 P53 和 RB 基因突变与不同 BC 组织学类型之间观察到统计学显著关联。另一方面，HPV 基因分型与不同 BC 分级、BC 组织学类型、P53 和 RB 基因突变以及雌激素和孕激素受体表达之间无统计学显著关联。此外，雌激素和孕激素受体表达与 BC 分级之间无统计学显著关联。RB 基因突变与不同 BC 分级显著相关，而孕激素受体表达与 BC 无统计学显著关联。
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